IN-VITRO ANTIBACTERIAL AND CYTOTOXICITY EVALUATION OF SOME NOVEL TETRAZOLE DERIVATIVES
HTML Full TextIN-VITRO ANTIBACTERIAL AND CYTOTOXICITY EVALUATION OF SOME NOVEL TETRAZOLE DERIVATIVES
Anis Ahamed 1, Ibrahim A. Arif 1, Meera Moydeen 2, Radhakrishnan Surendra Kumar 3 and Akbar Idhayadhulla * 3
Department of Botany and Microbiology 1, Prince Sultan Research Chair for Environment and Wildlife, Department of Chemistry 2, College of Sciences, King Saud University (KSU), Riyadh, Saudi Arabia.
Department of Chemistry 3, Nehru Memorial College (Affiliated to Bharathidasan University), Puthanampatti, Tiruchirappalli - 621007, Tamil Nadu, India.
ABSTRACT: A series of tetrazol derivatives 1(a-c) and 2(a-c) were synthesized by Mannich base reaction. Synthesized compounds 1(a-c) and 2(a-c) were confirmed by IR, ¹H NMR, ¹³C NMR, mass spectral, and elemental analysis. Synthesized compounds 1(a-c) and 2(a-c) were screened for dental plaque bacteria and cytotoxicity activity. The compound 1b was highly active against Enterococcus feacalis in antibacterial screening. The synthesized compounds have been screened for preliminary cytotoxicity against HepG2 (Liver), Hela (Cervical) and MCF-7 (Breast) cancer cells. The compound (1c) is highly active against MCF-7(Breast), and compound (2b) is highly active against HepG2 (Liver). Therefore, current study demonstrates the antibacterial and cytotoxicity activity potential of new tetrazole derivatives and provides future insights for developing dental plaque antibacterial drugs.
Keywords: |
Tetrazole derivatives, Dental plaque bacteria, Cytotoxicity screening
INTRODUTION: Azoles (imidazole, triazole and tetrazole) are presented in many effective anti-microbial activities and they are widely used for the treatment of topical or inner mycoses in particular AIDS-related mycotic pathologies 1. Tetrazoles have been used as high energy compounds and some of the tetrazoles are used as drugs. A few examples are shown in Fig. 1. Losartan (1) is an angiotensin II antagonist and commonly used for treatment of hypertension. Tetrazole (2) has also been found to posses binding affinity to benzo- diazepine receptors 2.
Mannose mimetics (3) have been reported to be inhibitors of α-mannosidase 3, 4. 1,5-Disubstituted-1H-tetrazoles (4) is suitable bioisosteres of peptides 5, some of tetrazole derivatives are medicinally importance and it’s have been reported to possess antibiotics 6 antifungal drugs 7, antinociceptive 8, 9, anti-mycobacterial 10, anti-inflammatory 11, anti-proliferative 12 and anticonvulsant activities 13.
FIG. 1: BIOLOGICAL ACTIVE TETRAZOLES DERIVATIVES
Mannich reaction is of considerable importance for the synthesis of multidrug compounds and biologically important compounds 14 - 15. Basically Mannich bases have several biological activities such as antimicrobial 16, 17, 18, cytotoxic 19 and anticonvulsant activities 20. Therefore, bearing in mind the above observation, we decided to synthesize new series of tetrazol derivatives and screening for dental plaque antibacterial and cytotoxicity activities.
MATERIALS AND METHODS:
Chemistry: Melting points were recorded in open capillary tubes and were uncorrected. The IR spectra were recorded in KBr on an FT-IR spectrometer (Shimadzu 8201PC) in the range of 4000-400 cm-1. The 1H NMR spectra were recorded on a Bruker DRX-300 spectrometer at 300MHz. Elemental analysis (C, H, N and S) were performed using an elemental analyzer (Vario EL III). The purity of the compounds was checked by thin layer chromatography (TLC) on silica gel plates.
Synthesis of 2-[phenyl(1H-tetrazol-1-yl)methyl] hydrazinecarbothioamide 1(a-c): A mixture of tetrazole (0.1 mol, 7.0 g), thiosemicarbazone (0.1 mol, 9.1g) and benzaldehyde (0.1 mol, 10mL) in ethanol (30 mL), the reaction mixture was taken in RB flask. The reaction mixture was refluxed and stirred for 2h with help of magnetic stirrer. Final product was purified by column chromatography.
2-[phenyl(1H-tetrazol-1-yl)methyl]hydrazinecar bothioamide 1(a): FT-IR (KBr, cm-1): 3408 (NH2), 3002 (NH), 2926 (CHstr), 1660 (C=S), 1512 (N=N), 1315 (C=N), 947(NH), 701(ArH). ¹H NMR (DMSO-d6), δH (ppm): 9.60 (2H, s, NH2), 8.72 (1H, s, 5CH-tetrazole), 7.30-7.26 (5H, m, Ph), 6.32 (1H, s, -CH-), 2.22(1H, s, NH). 13C NMR (DMSO-d6), δC (ppm): 182.86(C=S), 144.87 (5CH-tetrazole), 138.26-126.67 (Ph), 74.11(-CH-). EI-Ms, m/z (Relative intensity %): m/z 249.09 (M,+ 10%).
2-[(4-chlorophenyl)(1H-tetrazol-1-yl) methyl] hy drazinecarbothioamide 1(b): FT-IR (KBr, cm-1): 3377 (NH2), 3023(NH), 2932 (CHstr), 1653(C=S), 1577(N=N), 936(NH), 646(Ar-Cl). ¹H NMR (DMSO-d6) δH (ppm): 9.52(NH2,s,2H), 8.82(5CH-teterzole, s,1H), 7.75(2H, dd, Ph, J=5.6Hz, J=6.2Hz), 7.44 (2H, dd, Ph, J=5.8Hz, J=6.4Hz), 6.43(1H, s, -CH-), 2.43(1H, s, NH), 2.14 (1H, s, NH). 13C NMR (DMSO-d6), δ(ppm): 181.12(C=S), 147.67 (5CH-tetrazole), 131.23 (C-Cl), 130.12-129.11 (Ph), 72.11 (-CH-). EI-Ms, m/z (Relative intensity %): m/z 283.65 (M,+ 66%).
2-[(4-hydroxyphenyl)(1H-tetrazol- 1 -yl) methyl] hydrazinecarbothioamide 1(c): FT-IR (KBr, cm-1): 3408 (NH2), 2997(NH), 2909 (CHstr) 1560 (N=N), 1660 (C=S), 1315 (C=N), 942 (NH), 942 (Ar-OH) . ¹H NMR (DMSO-d6), δH (ppm): 9.83 (1H, s, Ph-OH), 9.21 (2H, s, NH2), 8.36 (1H, s, 5CH-tetrazole), 7.43(2H, dd, Ph, J=6.8Hz, J=7.2Hz), 7.22(2H, dd, Ph, J=6.7Hz, J=7.0Hz), 6.32(1H, s, -CH-), 2.43(1H, s, NH). 13C NMR (DMSO-d6), δC (ppm): 180.21 (C=S), 154.07(Ph-OH), 143.67 (5CH-tetrazole moiety), 138.26-126.67 (Ph), 72.08 (-CH-). EI-Ms, m/z (Relative intensity %): m/z 264.98(M,+ 26%); .
Synthesis of 1, 1-dimethyl-3-[phenyl (1H-tetra zol-1-yl) methyl] urea 2(a-c): A mixture of tetrazole (0.1mol, 7.0g), 1, 1-dimethylurea (0.1mol, 8.8g) and benzaldehyde (0.1 mol, 10mL) in ethanol (30 mL), the reaction mixture was taken in RB flask.
The reaction mixture was refluxed and stirred for 2h with help of magnetic stirrer. The reaction mixture cooled and poured into crushed ice. The resulting solid was filtered, dried and recrystallized from ethanol.
1,1-dimethyl-3-[phenyl(1H-tetrazol-1-yl) methyl] urea 2(a): FT-IR (KBr, cm1): 2986 (NH), 2936 (CHstr), 1694 (C=O), 1512 (N=N), 1376 (C=N), 942 (NH), 919 (Ar). ¹H NMR (DMSO-d6) δH (ppm): 9.52(2H, s, NH2), 8.82 (2H, s, 5CH-tetrazole), 7.75-7.44 (5H, m, Ph), 6.43 (1H, s, -CH-), 6.12 (1H, s, NH), 2.14 (6H, s, -NH(CH3)2). 13C NMR (DMSO-d6), δ (ppm): 154.07 (C=O), 147.67 (5CH-tetrazole), 137.86-126.21 (Ph), 72.11(-CH-), 36.12 (-N(CH3)2). EI-Ms, m/z (Relative intensity %):m/z 246.88(M,+ 44%).
3-[(4-chlorophenyl)(1H-tetrazol-1-yl)methyl]-1,1 -dimethylurea 2(b): FT-IR (KBr, cm-1): 3240 (CH3), 2934 (CHstr), 1683(-CONH), 1565(N=N), 1363 (C=N), 954(Ar), 646(Ar-Cl). ¹H NMR (DMSO-d6) δH (ppm): 8.85 (1H, s, 5CH-teterzole), 7.47(2H, dd, Ph, J=5.9Hz, J=6.8Hz), 7.26 (2H, dd, Ph, J=5.7Hz, J=6.6Hz), 7.15(1H, s, -CH-), 6.13 (1H, s, NH), 2.83(6H, s, -NH(CH3)2). 13C NMR (DMSO-d6), δ (ppm): 156.67 (C=O), 143.30 (5CH-teterazole), 132.06(C-Cl), 131.01- 128.30 (Ph), 66.21 (-CH-), 36.12 (-N(CH3)2). EI-Ms, m/z (Relative intensity %): m/z 279.12(M,+ 38%).
3-[(4-hydroxyphenyl)(1H-tetrazol-1-yl) methyl] -1,1-dimethylurea 2(c): FT-IR(KBr, cm-1): 3297 (CH3), 2922 (CHstr), 1683 (-CONH), 1565 (N=N), 1377 (C=N), 942 (Ar), 942 (Ar-OH),. ¹H NMR (DMSO-d6) δH (ppm): 9.83 (1H, s, Ph-OH), 8.84 (1H, s, 5CH-teterzole), 7.31(2H, dd, Ph, J=6.3Hz, J=5.7Hz), 7.22 (2H, dd, Ph, J=6.6Hz, J=5.8Hz), 7.14(1H, s, -CH-), 6.13 (1H, s, NH2), 2.81(6H, s, -NH(CH3)2). 13C NMR (DMSO-d6), δC (ppm): 154.07 (Ph-OH), 156.43 (C=O), 143.12 (5CH-teterazole), 131.42-128.09 (Ph), 66.09 (-CH-), 36.01 (-N(CH3)2). EI-Ms, m/z (Relative intensity %): m/z 262.87 (M,+ 21%).
In-vitro Dental Plaque Antibacterial Screening: The compounds 1(a-c) and 2(a-c) were evaluated against Staphylococcus aureus, Escherichia coli, Enterococcus feacalis, Pseudomonas aeruginosa, and Klebsiella pneumoniae (recultured) by disc diffusion method 21, 22 was performed using Mueller-Hinton agar (Hi-Media) medium. Each compound was tested at a concentration at 50 and 100 μg/mL in DMSO. The zone of inhibition was measured after 24h incubation at 37 ºC.
Determination of the Minimal Inhibitory Concentration (MIC): Compound was dissolved in dimethylsulphoxide at concentration of 64 µg/mL. The two fold dilutions of the solution were prepared (64, 32, 0.5 µg/mL). The microorganism suspensions at 106 CFU/mL (colony forming unit/mL) concentrations were inoculated to the corresponding wells. The plates were incubated at 36º C at 24 h.
Cytotoxic Activity: The newly synthesized compounds 1(a-c), and 2(a-c) were screened for their cytotoxicity activity according to the procedure suggested 23.
RESULTS AND DISCUSSION:
Chemistry: A series of compounds 1(a-c), and 2(a-c) were synthesized from condensation method and reactions are outline in Fig. 2, physicochemical data are given in Table 1.
TABLE 1: PHYSICAL CHARACTERIZATION OF COMPOUNDS 1(a-c), 2(a-c)
Com.
no. |
R | m. w. | Yield
(%) |
M. F | Elemental analysis | Calculated
(Found) (%) |
Elemental Analysis | Calculated (Found) (%) |
C | H | N | S | |||||
1a | -H | 249.29 | 82 | C9H11N7S | 43.36 (54.70) | 4.45 (5.14) | 39.33 (18.79) | 12.86 (12.97) |
1b | -Cl | 265.29 | 79 | C9H11N7OS | 40.75 (40.23) | 4.18 (4.22) | 36.96 (36.80) | 12.09 (12.20) |
1c | -OH | 283.70 | 81 | C9H10N7SCl | 38.10 (52.41) | 3.55 (3.91) | 34.56 (34.61) | 11.30 (11.47) |
2a | -H | 233.23 | 78 | C9H11N7O | 46.35 (46.34) | 4.75 (4.71) | 42.04 (42.06) | - |
2b | -Cl | 246.12 | 86 | C11H14N6O | 53.65 (53.60) | 5.73 (5.71) | 34.13 (34.10) | - |
2c | -OH | 210.87 | 80 | C11H14N6O2 | 50.38 (50.29) | 5.38 (5.35) | 32.04 (32.10) | - |
The structures of compounds were characterized from IR, ¹H NMR, ¹³C NMR and Mass spectral analysis. IR spectra of the compounds (1a) shows that the absorption band at NH2, C=S, C=N and N=N corresponding to 3408, 1660, 1315, and 1512 cm-¹ respectively. ¹H NMR spectrum of the compound (1a) shows that signals obtained at δ 9.60, 6.32, and 2.22 corresponding to NH2, -CH- and NH respectively.
The ¹³C NMR spectrum of the compound (1a) shows that signals obtained at δ 182.86 and 74.11 corresponding to (C=S) and -CH- carbon group respectively. Mass spectra of the compound (1a) shows molecular ion peak at m/z 249.09 corresponding to expected molecule weight of the compound (1a). IR spectra of the compound (2a) shows that the absorption band at C=O, C=N, and NH corresponding to 1694, 1376, and 942 cm-1 respectively. ¹H NMR spectrum of the compound (2a) shows that the proton signals observed 6.43, 9.52, 6.12, and 2.14 corresponding to -CH-, NH2, NH, and (-NH(CH3)2 protons respectively.
FIG. 2: SYNTHETIC ROUTE OF THE COMPOUNDS 1(a-c) AND 2(a-c)
The ¹³C NMR spectrum of the compound (2a) shows that the carbon peaks obtained at 154.07, 72.11 and 36.12 corresponding to C=O, CH, and CH2N respectively. Mass spectra of the compound (2a) shows molecular ion peak at m/z 246.88 corresponding to expected molecule weight of the compound (2a).
Biological Screnning:
Antibacterial Activity: The compounds 1(a-c), and 2(a-c) were screened for antibacterial activity. The compound (1b) was highly active (MIC: 8 µg/mL) against E. faecalis compared with standard and the compound (2b) has highly active (MIC: 8 µg/mL) against E. coli compared with other compounds completely remove this sentence. The compound (1c) was highly active against S. aureus (MIC: 4µ/mL) compound with other compounds but very low active compared with standard ciprofloxacin. The values are summarized in Table 2, antibacterial minimum inhibit concentration value are summarized in Table 3.
TABLE 2: ANTIBACTERIAL ACTIVITYOF COMPOUNDS 1(a-c), 2(a-c). ZONE OF INHIBITION IN mm
Com.
no. |
S.
aureus |
E.
coli |
E.
faecalis |
P. aeruginosa | K.
pneumoniae |
1a | 10 | 12 | - | - | 14 |
1b | 12 | 19 | 22 | 16 | 18 |
1c | 20 | 10 | 17 | 10 | 8 |
2a | 14 | 17 | 10 | 10 | 16 |
2b | 17 | 22 | 10 | 12 | 18 |
2c | 10 | 13 | 10 | 19 | 10 |
Standard | 22 | 26 | 16 | 30 | 18 |
TABLE 3: THE MINIMAL INHIBITORY CONCENTRATIONS (MIC, µg/mL) OF COMPOUNDS 1(a-c), 2(a-c) AGAINST ORAL BACTERIAL SPECIES
Com. no. | SA | EC | EF | PA | KP |
1a | 64 | 64 | - | - | 32 |
1b | 64 | 8 | 8 | 32 | 16 |
1c | 4 | 32 | 16 | 64 | >100 |
2a | 64 | 64 | >100 | >100 | 64 |
2b | 16 | 8 | >100 | 64 | 16 |
2c | 64 | 32 | >100 | 16 | >100 |
Ciprofloxacin | 0.5 | 0.5 | 16 | 0.5 | 2 |
Oral bacterial species, zone of inhibition measured at (mm).
The compounds were used at concentration 100 µg/mL. Ciprofloxacin used as a standard
Cytotoxicity: Compounds 1(a-c), 2(a-c) were found to be active in the preliminary cytotoxicity screening studies. The compounds were tested against the three cell lines of liver, cervical, breast cancer types. Their GI50, TGI and LC50 values were determined. The result of the screening was expressed in terms of GI50 growth inhibitor concentration.
Table 4 shows that the compound (1c) has highly active against MCF7 cancer cell line for the reason that low growth of inhibition (GI50) at 8.2 μm compared to other and compounds (2b) is highly active against HepG2 for the reason that low Growth of inhibition (GI50) at 5.4μm compared to other and compounds.
Structure Activity Relationship: From the results of antimicrobial and cytotoxicity activities, we are discussed in following structure activity relationships:
TABLE 4: CYTOTOXICITY ACTIVITY OF COMPOUNDS 1(a-c), 2(a-c)
Compounds
|
HepG2 | MCF-7 | HeLa | ||||||||
GI50 | TGI | LC50 | GI50 | TGI | LC50 | GI50 | TGI | LC50 | |||
1a | 16.2 | 29.1 | >100 | 22.9 | 46.8 | >100 | 21.6 | 49.4 | 81.2 | ||
1b | 23.3 | 54.8 | 81.2 | 20.1 | 45.1 | >100 | 41.0 | 87.2 | >100 | ||
1c | 18.2 | 58.1 | 90.1 | 8.2 | 16.1 | 57.2 | 20.2 | 48.1 | 84.1 | ||
2a | 6.3 | 15.3 | 51.2 | 5.2 | 20.1 | >100 | 8.1 | 17.1 | 65.3 | ||
2b | 5.4 | 12.5 | 62.5 | 13.5 | 26.9 | 83.5 | 16.8 | 34.7 | 92.8 | ||
2c | 31.7 | 62.1 | >100 | 22.6 | 52.5 | 88.4 | 29.8 | 52.6 | >100 |
FIG. 3: STRUCTURE ACTIVE RELATIONSHIP ACTIVE COMPOUND
Fig. 3 indicates that highlighted that structure activity relationship. The compound (1c) is highly active against S. aureus (MIC, 4 µg/mL) as well as the compound response to MCF-7 cancer cell lines corresponding to TGI 16.1 due to presence of tetrazole ring with hydroxybenzene. The compound (2b) is highly active against E.coli (MIC, 8.µg/mL) as well as compound response to HepG2 (Liver) cancer cell line corresponding to TGI 12.5, due to presence of tetrazole ring with chlorophenzen.
CONCLUSION: In conclusion, we have found an efficient and practical procedure for the synthesis of tetrazole derivatives. The compound (1b) was highly active against Enterococcus feacalis in antibacterial screening. The most of compounds were active due to the para substitution of phenyl ring with tetrazole ring in antibacterial screening. The compound (1c) showed significant cytotoxicity properties against MCF7 cancer cell line and compound (2b) showed significant cytotoxicity properties against HepG2 cancer cell line with GC50 values in micromolar range.
Overall, this study demonstrates that antibacterial and cytotoxicity activity potential of new tretazole derivatives and provides future insights for developing dental plaque antibacterial drugs.
ACKNOWLEDGEMENT: The project was supported by King Saud University, Deanship of Scientific Research Chair. We are very grateful to Prince Sultan Research Chair for Environment and Wildlife and Saudi Biological Society. We thank the Department of Botany and Microbiology, College of Sciences, King Saud University (KSU), Riyadh, Saudi Arabia for encouragement and support for funding this work.
CONFLICT OF INTEREST: The authors have declared no conflict of interest.
REFERENCES:
- Koltin Y: Targets for antifungal drug discovery. Annual Reports in Medicinal Chemistry Chapter 15. 1990; 25: 141-48.
- Daya S, Kaye PT and Mphahlele MJ: Benzodiazepine analogues. part 12.An investigation of substituent and ring-atom effects on receptor binding affinities. Medical Science Research 1996; 24: 137-41.
- Davis B, Benjamin G. Nash DRJ, Watson AA, Smith C, and Fleet GWJ: Tetrazoles of manno-and rhamno-furanoses. Tetrahedron Letters 1995; 36: 7507-510.
- Brandstetter TW, Brandstetter TW, Davis B, Hyett D, Smith C, Hackett L, Winchester BG and Fleet GWJ: Tetrazoles of manno- and rhamno-pyranoses: inhibition of Glycosidases by tetrazoles and other mannose mimics. Tetrahedron Letters 1995; 36: 7511-14.
- Zabrocki J: Conformationalmimicry. 1. 1,5-Disubstituted tetrazole ring as surrogate for the cis amide bond. Journal of the American Chemical Society 1988; 110: 5875-80.
- Feinn L, Dudley J, Coca A and Roberts EL: Antimicrobial evaluation of 5-substituted aryl 1H-tetrazoles. Medicinal Chemistry 2017; 13: 359-64.
- Qian A, Zheng Y, Wang R, Wei J, Cui Y, Cao X and Yang Y: Design, synthesis and structure- activity relationship studies of novel tetrazole antifungal agents with potent activity, broad antifungal spectrum and high selectivity. Bioorganic and Medicinal Chemistry Letters 2017; 28: 344-50.
- Silva DP, Florentino IF, Oliveira LP, Lino RC, Galdino PM, Menegatti R, and Costa EA: Anti-nociceptive and anti-inflammatory activities of 4-[(1-phenyl-1H-pyrazol-4-yl) methyl] 1-piperazine carboxylic acid ethyl ester: A new piperazine derivative. Pharmacology Biochemistry Behavior 2015; 137: 86-92.
- Rajasekaran A and Thampi PP: Synthesis and anti-nociceptive activity of some substituted-{5[2-(1,2,3,4-tetrahydrocarbazol-9-yl)ethyl]tetrazol-1-yl}alkanones. European Journal of Medicinal Chemistry 2005; 40(12): 1359-64.
- Karabanovich G, Roh J, Smutný T, Němeček J, Vicherek P, Stolaříková J, Vejsová M, Dufková I, Vávrová K, Pávek P, Klimešová V and Hrabálek A: 1-Substituted-5-[(3,5-dinitrobenzyl)sulfanyl]-1H-tetrazoles and their isosteric analogs: A new class of selective antitubercular agents active against drug-susceptible and multidrug-resistant mycobacteria, European Journal of Medicinal Chemistry 2014; 82: 324-40.
- Lamie PF, Philoppes JN, Azouz AA and Safwat NM: Novel tetrazole and cyanamide derivatives as inhibitors of cyclooxygenase-2 enzyme: design, synthesis, antiinfla -mmatory evaluation, ulcerogenic liability and docking study. Journal of Enzyme Inhibition and Medicinal Chemistry, 2017; 32: 805-20.
- Romagnoli R, Baraldi PG, Salvador MK, Preti D, Tabrizi MA, Brancale A, Fu XH, Li J, Zhang SZ, Hame E, Bortolozzi R, Basso G, and Viola G: Synthesis and evaluation of 1,5-disubstituted tetrazoles as rigid analogues of combretastatin A-4 with potent anti- proliferative and antitumor activity, Journal of Medicinal Chemistry 2012; 55 (1): 475-88.
- Dong S, Wang T, Wang H, Qian K, Zhang Z, Zuo Y, Luo G, Jin Y and Wang Z: Synthesis and evaluation of 5-(o-Tolyl)-1H-tetrazole derivatives as potent anticonvulsant agents. Archiv der Pharmazie 2017; 350: 1-5.
- Tramontina M: Advances in the chemistry of mannich bases. Synthesis 1973; 12: 703-75.
- Tramontini M and Angiolini L: Further advances in the chemistry of mannich bases. Tetrahedron 1990; 46: 1791-37.
- Popiołek L, Biernasiuk A, Paruch K, Patrejko P and Wujec M: Synthesis and evaluation of antimicrobial properties of new Mannich bases of 4,5-disubstituted-1,2,4-triazole-3-thiones, Phosphorus, Sulfur, and Silicon and the Related Elements 2017; 192: 880-85.
- Al-Abdullah ES, Al-Tuwaijri HM, Hassan HM, Haiba ME, Habib EE and El-Emam AA: Antimicrobial and hypoglycemic activities of novel N-Mannich bases derivedfrom 5-(1-adamantyl)-4-substituted - 1, 2, 4-triazoline-3-thiones, International Journal of Molecular Sciences 2014; 15(12): 22995-3010.
- Idhayadhulla A, Surendra Kumar R, JamalAbdul Nasser A, Selvin J and Manilal A: Synthesis of some mannich base derivatives and their antimicrobial activity study. Arabian Journal of Chemistry 2014; 7: 994-99.
- Bhupendra MM, Shin HS, Keum YS, Pandurangan M, Kim DH, Moon SH, Kadam AA, Shinde SK and Patel RV: Synthesis and evaluation of antioxidant and cytotoxicity of the n-mannich base of berberine bearing benzothiazole moieties, Anti-Cancer Agents in Medicinal Chemistry 2017; 17: 1652-60.
- Rybka S, Obniska J, Rapacz A, Filipek B and Żmudzki P: Synthesis and evaluation of anticonvulsant properties of new N-Mannich bases derived from pyrrolidine-2,5-dione and its 3-methyl-, 3-isopropyl, and 3-benzhydryl analogs. Bioorganic and Medicinal Chemistry Letters 2017; 27: 1412-15.
- Kumar SR, Ibrahim AA, Anis A and Idhayadhulla A: Anti-inflammatory and antimicrobial activities of novel pyrazole analogues. Saudi Journal of Biological Sciences 2016; 23: 614-20.
- Meera M, Al-Deyab SS, Kumar SR and Idhayadhulla A: Efficient synthesis of novel 3-phenyl-5-thioxo-3,4,5,6-
- tetrahydroimidazo [4,5-c] pyrazole - 2(1H) carbothioamidederivatives using a CeO2–MgO catalyst and evaluation of antimicrobial activity, Journal of Heterocyclic Chemistry 2017; 54: 3208-19.
- Surendra Kumar R, Moydeen M, Al-Deyab SS, and Manilal A, Idhayadhulla A: Synthesis of new morpholine-connected pyrazolidine derivatives and their antimicrobial, antioxidant and cytotoxic activities. Bioorganic and Medicinal Chemistry Letters 2017; 27: 66-71.
How to cite this article:
Ahamed A, Arif IA, Moydeen M, Kumar RS and Idhayadhulla A: In-vitro antibacterial and cytotoxicity evaluation of some novel tetrazole derivatives. Int J Pharm Sci Res 2018; 9(8): 3322-27. doi: 10.13040/IJPSR.0975-8232.9(8).3322-27.
All © 2013 are reserved by International Journal of Pharmaceutical Sciences and Research. This Journal licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
Article Information
23
3322-3327
404
961
English
IJPSR
A. Ahamed, I. A. Arif, M. Moydeen, R. S. Kumar and A. Idhayadhulla *
Department of Chemistry, Nehru Memorial College, Puthanampatti, Tiruchirappalli , Tamil Nadu, India.
a.idhayadhulla@gmail.com
19 November, 2017
16 February, 2018
19 February, 2018
10.13040/IJPSR.0975-8232.9(8).3322-27
01 August, 2018