IN-VITRO ANTIDIABETIC ACTIVITY OF 2-(3,4-DIHYDROXYPHENYL)-3,5,7-TRIHYDROXY-4H-CHROMEN-4-ONE ISOLATED FROM THE METHANOLIC EXTRACT OF ANDROGRAPHIS ECHIOIDES LEAVES

In the present study the 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one  isolated from the methanolic extract of leaves of Andrographis echioides was studied for alpha-amylase and alpha-glucosidase inhibition using an in-vitro model. The isolation was done using column chromatography using gradient elution with different mobile phase. Structural elucidation was carried out on the basis of spectral analysis. The study revealed that the 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC₅₀ value of 36.2 ± 0.42 and 41.4 ± 0.34% respectively and well compared with standard acarbose drug. The infra-red spectrum specific absorption bands for 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one viz. 3401.71 (O-H stretching vibration of phenol), 1666.95 (C=O Aryl ketonic stretch), 1612.42 (C-C Aromatic ring stretch), 1519.96 (C=O aromatic stretch), 1463.09 (C=C aromatic stretch), 1380.89 (O-H bending of phenols), 1318.98 (C-H bond in Aromatic hydrocarbon), 1246.04 (C-O stretch of Aryl ether), 1213.26 (C-O stretch of phenol), 1169.06 (C-CO-C stretch and bending in ketone), 932.62, 807.89, 702.92, 638.65 (C-H bending of aromatic hydrocarbons). Structural elucidation of 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one was done by spectrum analysis such as ¹³C and ¹H depth nuclear magnetic resources. Mass spectra of 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one showed a parent molecular ion [M+] peak at m/z 303 which corresponds to molecular formulae C₁₅H₁₀O₇. Therefore, it is suggested that 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-chromen-4-one is a potential source for natural antidiabetic compounds and could have potential use in the management of diabetes mellitus.