IN VITRO ANTIOXIDANT ACTIVITIES AND CYTOTOXICITY STUDY OF THE METHANOLIC EXTRACT OF BARKS OF SYZYGIUM CYMOSUM
AbstractThe aim of the study to determine antioxidant and cytotoxic properties of the bark extracts of Syzygium cymosum along with phytochemical study for the presence of phytochemical constituents. Total phenolic and flavonoid content of the S.Cymosum extracts was determined by using the Folin- Ciocalteu reagent and aluminum chloride (AlCl3) method, respectively. The crude methanolic extract of S.Cymosum was studied to evaluate its antioxidant activity by DPPH radical scavenging assay and reducing power assay. Brine Shrimp Lethality Bioassay was done for cytotoxic activity. Bark extracts of S.Cymosum have been shown to possess phytoconstituents including carbohydrates, alkaloid, glycosides, steroids, tannins and saponin. The extract was found to contain significant amount of phenol and flavonoid in Folin-Ciocalteau and total flavonoid content assay. Total antioxidant capacity of the extract was estimated to be 505.5±3.535 mg/g of ascorbic acid equivalent. Its IC50 value in DPPH method was 64.99079µg/ml. The extract showed concomitant increase in reducing power with the increase of concentration of the extract. In case of brine shrimp cytotoxicity assay, LC50 value was obtained to be 307 μg/ml. Data from present results revealed that it act as an antioxidant agent due to its free radical scavenging and it has also cytotoxic activity
Article Information
16
1021-25
373
1306
English
IJPSR
Kakan Sutra Dhar, Tania Binte Wahed *, A. H. M. Nazmul Hasan and Sonia Binte Wahed
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, Bangladesh
tania.wahed@yahoo.com
09 September, 2015
15 December, 2015
05 January, 2016
10.13040/IJPSR.0975-8232.7(3).1021-25
01 March, 2016