IN-VITRO ANTIOXIDANT, ANTIBIOTIC COMPLEMENTARY OR SUPPLEMENTARY EFFECTS AND BACTERICIDAL ACTIVITIES OF THE SEEDS OF A WEED: HYGROPHILA AURICULATA (K. SCHUM) HEINE AGAINST UROPATHOGENS

Upgradation of conventional medications with additional therapeutic phytocompounds to combat emerging resistant pathogenic strains, is uptrend now-a-days. The current work investigates the precise in-vitro bactericidal efficacy of the methanolic extract of the unexplored seeds of a swampy weed, Hygrophila auriculata (K. Schum) Heine (MHA), Family – Acanthaceae against different uropathogens. It was observed that, 200µg of MHA has 20% of supplementary inhibitory effect on the antibiotic disc of Amoxicillin-clavulanic acid 30 against Enterococcus faecalis and Escherichia coli. Similarly, MHA treated Nitrofurantoin 300 disc shows 25%, 4.5% and 5% of supplementary inhibitory effect against E. faecalis, E. coli and Staphylococcus aureus, respectively. Ciprofloxacin 5 is supplemented by 2.8% and Streptomycin 10 by 4.5% against S. aureus. 39.28%, 42.39%, 53.36% and 90.4% of increased inhibition was recorded against E. faecalis, E. coli, Pseudomonas aeruginosa and S. aureus respectively in terms of cfu/mL, on treating with a higher concentration of MHA. Present study has noted a minimum inhibitory concentration of 2.5mg/mL of MHA each against E. coli, P. aeruginosa and 1.25mg/mL against E. faecalis, Proteus vulgaris and S. aureus, respectively. Alkaloids, carbohydrates, coumarin, flavonoids, phenols, proteins, steroids, and tannins were tested as the likely bioactive components for the antibacterial response. The phenol, flavonoid content and IC₅₀ value of MHA is quantified as 20.42 ±1.2 mg/g of Gallic acid equivalent, 348 ± 8.6 mg/g of Rutin equivalent and 58.37µg/mL, respectively to potentiate its antioxidant capacity. Our findings imply warranted development of potent antibacterial agent from the MHA in pharmaceutical sector.