IN-VITRO ANTIVIRAL ACTIVITY OF INDIAN MEDICINAL PLANTS TO ASIAN AND EAST CENTRAL SOUTH AFRICAN LINEAGE OF CHIKUNGUNYA VIRUSAbstract
Indian medicinal plants traditionally used for the treatment of viral infections with high fever, arthritis, inflammation were investigated for their in vitro antiviral activity against the Asian and East Central South African lineage of Chikungunya virus. In vitro cytotoxic assay of aqueous, aqueous ethanolic and ethanolic extracts of Alpinia Galanga (L) Willd., Alpinia Officinarum Hance., Andrographis paniculata Wall. ex. Nees , Melia azedarach L., and Azadirachta indica A. Juss were assessed for the determination of maximal nontoxic concentration in Vero cell lines. Antiviral activity of aqueous, aqueous ethanolic and ethanolic extracts of plants were determined by the inhibition of virus induced cytopathic assay to both lineages of Chikungunya virus aqueous ethanolic and ethanolic extracts of Melia azedarach L. were found to be effective and inhibited the growth of Chikungunya virus. Asian strain of Chikungunya virus was found to be more susceptible at lower concentrations of the extracts tested than the ECSA strain.
K. Sangeetha and S. Rajarajan*
PG & Research Dept of Microbiology & Biotechnology, Presidency College (Aut), Chennai, TN, India
21 June, 2014
27 August, 2014
29 October, 2014
01 February, 2015