IN-VITRO CYTOTOXIC ACTIVITY OF SQUID AND CUTTLEFISH BONE EXTRACT ON HEP G2 CELL LINEAbstract
The present study was carried out to assess the anticancer activity of methanol extracts of internal bone of L.duvauceli and S. pharaonis for cell viability and toxicity on HepG2 liver cancer cells. The bone extracts had significant cytotoxicity on HepG2 cell in different concentrations (100,500,1000 µg/ml). Methanol fraction of squid bone powder showed cell death at a higher concentration of 1000µg. 27% toxicity was observed at 100µg/ml and 37% at 1000µg/ml. The viability of cuttlefish bone powder ranged from 58% (1000µg/ml) to 68% (100µg/ml). Hep G2 cells experienced a significant decrease in viability at low concentration with an eventual decline at the highest concentration. The percentage of toxicity of cuttle bone extracts varied from 32 to 42. Minimum toxicity of 32% was observed at 100µg and maximum of 42% was observed at 1000µg concentration and IC 50 at a concentration of >1000µg. The percentage of toxicity showed increasing trend with increasing concentration of the extract. The result suggested that the cephalopods bone is a source to be considered in the discovery of drug development for cancer.
Jemma Hermelin Jesy Diaz*, R.D. Thilag and V. Sivakumar
Assistant Professor St. Mary’s College (Autonomous) Thoothukudi, Tamil Nadu, India.
23 April, 2014
24 June, 2014
16 January, 2015
01 February, 2015