IN-VITRO PROPAGATION OF TYLOPHORA OVATA (LIND.) HOOK. EX. STEUD – AN IMPORTANT MEDICINAL PLANT
AbstractEfficient in-vitro propagation of Tylophora ovata (Lind.) Hook. Ex. Steud was carried out in this present investigation. Nodal segments from the healthy grown plants were used as explants for culturing. Explants were cultured on standard Murashige and Skoog (MS) medium supplemented with different concentration of benzyl amino purine (BAP) and naphthalene acetic acid (NAA) for primary shoot proliferation. Remarkable shoot proliferation was observed in MS medium containing BAP (2.5 mg/L) and NAA (3.5 mg/L). For rooting of the micro shoot, half strength MS medium supplemented with indole acetic acid IAA (2 mg/L) and 3-indole butyric acid IBA (2.5 mg/L) showed the best result. After acclimatization and transplantation, 100% of the in-vitro shoot delivered was found healthy in ex vivo condition
Article Information
53
1083-1086
386KB
962
English
IJPSR
R. Jeyachandran* and M. Bastin
Department of Botany, St. Joseph’s College (Autonomous), Tiruchirappalli – 620 002, Tamil Nadu, India
jeyachandranrobert@gmail.com
18 October, 2013
12 December, 2013
16 February, 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.5(3).1083-86
01March2014
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