IN VITRO QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF ETHANOLIC AND 50% ETHANOLIC EXTRACTS OF TINOSPORA CORDIFOLIA, MOMORDICA CHARANTIA, CUCURBITA MAXIMA AND RAPHANUS SATIVUS

Phytochemicals are non-nutritive chemical constituents of plants which occur naturally in it such as Steroid, Terpenoids, Tannins, Carotenoids, Flavonoids, Alkaloids and Glycosides. The aim of the present study was to carryout qualitative and quantitative phytochemical analysis of ethanolic and 50% ethanolic extracts of Tinospora cordifolia (stems), Momordica chirantia (fruit), Cucurbita maxima (fruit) and Raphanus sativus (root). The result of the qualitative phytochemical screening revealed the presence of various secondary metabolites with highest activity in 50% ethanolic extracts of T. cordifolia, C. maxima and ethanolic extract of Tinospora cordifolia. Quantitative phytochemical analysis revealed that the total flavonoids content was found to be varied in different extracts wherein high activity was noted in 50% ethanolic extract of T.cordifolia (9.91 ± 1.46 mg CE/gm)followed by 50 % ethanolic extract of Cucurbita maxima (9.79 ± 0.35 mg CE/gm)and absolute ethanolic extract of T.cordifolia (9.75 ± 0.4 mg CE/gm) among all 8 tested extracts. Total phenol content was significantly high in 50% ethanolic extracts of Tinospora cordifolia (24.70 ± 2.04 mg GAE/gm) followed by 50% ethanolic extract of Cucurbita maxima (18.23 ± 0.03 GAE/gm) and absolute ethanolic extract of Tinospora cordifolia (12.33 ± 0.5mg GAE/gm).