IN VITRO STUDIES OF IMMUNOMODULATORY AND FREE RADICAL SCAVENGING ACTIVITIES OF FLAVONOID ISOLATED FROM MIMOSA PUDICAAbstract
Flavonoid isolated from Mimosa pudica was evaluated for its radical scavenging and immunomodulatory activities in-vitro. Aim of this study is to isolate flavonoid from Mimosa pudica and to determine its antioxidant and immunomodulatory properties. Flavonoid was isolated from dried samples of Mimosa pudica using soxhlet extraction in 80% methanol and purified by column chromatography. Various tests were done for the presence of flavonoid, and the isolate was confirmed as flavonoid by Thin Layer Chromatography (TLC) and Fourier Transform Infrared (FT-IR) analysis. Antioxidant activity of the isolated flavonoid was evaluated using assays such as the 1, 1‑diphenyl‑2‑picryl‑hydrazil (DPPH) radical scavenging assay and hydroxyl radical scavenging assay. In-vitro assay of immunomodulatory activity of flavonoid isolated from Mimosa pudica was done by using lymphocytes isolated from a human blood sample. The isolated flavonoid exhibited a dose-dependent enhancement in activity in DPPH assay and hydroxyl radical scavenging assays. In DPPH assay 10μg of ascorbic acid, equivalent activity was shown by 30μg of isolated flavonoid. The isolate exhibited strong DPPH radical scavenging activity with an IC50 value of 56.32 μg/ml as compared to the IC50 value of the reference standard, ascorbic acid 21.11μg/ml. In hydroxyl radical scavenging assay 100μg of quercetin equivalent activity was shown by 373.77μg of isolated flavonoid. Flavonoid isolated from Mimosa pudica showed in-vitro growth stimulatory effect on isolated normal lymphocytes, which was estimated by MTT assay. Percentages of the proliferation of lymphocytes at different concentrations were obtained. The results of the present study indicate that the flavonoid isolated from Mimosa pudica is a c
J. Jose, S. Sudhakaran *, A. T. Dhanya, T. M. S. Kumar, S. Jayaraman and E. J. Variyar
Department of Chemistry, School of Chemical Sciences, Swami Anandatheertha Campus, Payyanur, Kannur University, Kerala, India.
24 March 2014
30 April 2014
09 July 2014
01 October 2014