IN-VIVO EFFECT OF WITHACOAGULIN AND COAGULIN C ISOLATED FROM WITHANIA COAGULANS DUNAL FRUITS ON BLOOD PRESSURE OF ALBINO RATS

The present study was designed to evaluate the in vivo effect of withacoagulin and coagulin C isolated from Withania coagulans Dunal fruits on blood pressure of albino rats. DOCA (10 mg/kg; i.p.) along with diet high in sodium chloride salt was used to induce hypertension in rats. Comparative effects of withacoagulin and coagulin C isolated from Withania coagulans Dunal fruits were observed. Female Albino rats (100-120 g) were divided into seven experimental groups (n = 6). Normal control, Verapamil standard control (50 mg/kg, p.o.), withacoagulin treatment groups (25, 50 mg/kg; p.o.), coagulin C treatment groups (25, 50 mg/kg; p.o.) and Toxic control (DOCA with 2% normal saline ad libitum). Rats having more than 150 mm Hg systolic blood pressure were chosen for the treatment. Noninvasive tail blood pressure amplifier (NIBP200A) was used to measure the blood pressure of conscious rats at a fixed interval of time every day for four weeks. A significant decrease (P< 0.05) in the blood pressure of withacoagulin treated and the standard group was observed. Withacoagulin at dose 25 mg/kg produced significant (P<0.05) antihypertensive effect. Withacoagulin at 50 mg/kg dose produced a highly significant antihypertensive effect (P<0.001) as compared to verapamil. Coagulin C has been found to have no significant antihypertensive effect on albino rats.