IN -VIVO EVALUATION OF ABIES PINDROW AS AN ANTI-DIABETIC PLANT

Background: The present study was carried to evaluate the phytochemical, antioxidant, and anti-diabetic potential of Abies pindrow leaf extracts. Methods: The extracted leaf samples of Abies pindrow in methanolic and hydroalcoholic solutions were evaluated for phytochemical and antioxidant activities. In-vitro antioxidant activity of both test samples was calculated by DPPH radical scavenging capacity, reducing power activity, total phenolic and flavonoid contents. Further the study was carried to evaluate the anti-diabetic potential of hydroalcoholic extract of Abies pindrow leaves in Alloxan-induced diabetic rats for 21 days. Various biochemical parameters and histopathological studies were also carried out. Results: The hydroalcoholic extract of Abies pindrow was found to have more antioxidant potential than the methanolic extract. Also the same extract at high dose (400mg/kg) exhibited significant anti-diabetic activity than the low dose (200mg/kg) in alloxan-induced diabetic rats. The hydroalcoholic extract also showed improvement in parameters like body weight and lipid profile as well as regeneration of β-cells of the pancreas in alloxan-induced diabetic rats. Histopathological studies show reinforcement of the healing of pancreas by hydroalcoholic extract of Abies pindrow leaves, as a possible mechanism of their anti-diabetic activity. Conclusion: The results concluded that the plant extract was capable of managing diabetes and complications of diabetes. Hence may be considered as of the potential sources for the isolation of new oral anti-diabetic agents.