IN-VIVO PLATELET ANTI-AGGREGATION ACTIVITY OF CRUDE FUCOIDAN OF SARGASSUM POLYCYSTUM

Platelet or thrombosis is one of the most important factors in blood clots formation. However, activation of platelet aggregation plays a significant role in homeostasis process, but in excess, it causes different cardiovascular diseases such as myocardial infarction, atherothrombosis disease, and coronary artery disease. To reduce the occurrence of these diseases, anti-platelet agents can be used to prevent blood clots formation. Therefore, this study was conducted to test the antiplatelet activity of crude Fucoidan from the brown seaweed (Sargassum polycystum) on mice. The measurement parameters used included bleeding time, coagulation time and decrease in plasma uptake by the addition of ADP as a platelet aggregation agent. The mice used were divided into 5 groups: normal control group (0.1% Na-CMC suspension), positive control group (clopidogrel 75 mg/kg), and the crude Fucoidan doses of 50 mg, 100 mg, and 200 mg. The results showed that positive control and crude Fucoidan with doses of 50 mg, 100 mg, and 200 mg groups could prolong coagulation time and bleeding time and also increase the decrease in plasma uptake after ADP addition. Significant differences (p>0.05) were also observed with normal controls but positive control of clopidogrel did not show any significant difference.