INDUCTION OF ROOTS AND IN-VITRO REGENERATION OF NOTHAPODYTES NIMMONIANA GRAHAM FROM EMBRYO CULTURES

The present study was aimed to develop the effective protocol for induction roots and in-vitro regenerated plants from embryo culture of Nothapodytes nimmoniana. Excised embryos were sterilized under the aseptic condition and cultured on L2 medium fortified with different concentration (0.5 – 3 mg/L) and the combination of auxins and cytokinins. Maximum shoot initiation was observed in BAP (2 mg/L) and Kin (1 mg/L) and in a combination of BAP (0.5 mg/L) and NAA (2 mg/L). A higher number of roots were observed when the embryo was inoculated on L2 medium supplemented with NAA (1 mg/L) and 2, 4-D (0.5 mg/L). Whereas, elongated roots were observed on L2 medium fortified with BAP/KIN (0.5 mg/L) and NAA (1 mg/L). The proliferation of multiple shoots from the nodal explants of in-vitro plantlets, when sub-cultured on MS medium supplemented with BAP (2 mg/L), TZD (0.2 mg/L) and L-glutamine (100 mg/L). The obtained plantlets were hardened in soil-rite and then transferred them to a mixture of soil, sand, and manure (1:1:1). In conclusion, our study demonstrates the in-vitro root induction and regeneration of Nothapodytes nimmoniana.