INVESTIGATION OF EFFECT OF PHOSPHOLIPIDS ON PHYSICAL AND FUNCTIONAL CHARACTERIZATION OF PACLITAXEL LIPOSOMES

The present study was assessed to determine the effect of various grades of phospholipids on liposomes. The various grades of synthetic phospholipids such as Phospholipon 90G^® (PL90G), Phospholipon 80H^® (PL80H) and Phospholipon 90H^® (PL90H) were used along with cholesterol in stoichiometric ratio (1:0.25, 1:0.5, 1:1, 1:2, 1:3). Using these ratios the paclitaxel liposome was formulated by film hydration method. The prepared liposomes were assessed for characterization using entrapment efficiency, vesicular size, in vitro release and hemolytic toxicity assay. The synthetic grades of phospholipids significantly influenced paclitaxel liposomal formulations. The stoichiometric ratio (1:1) between cholesterol and various synthetic phospholipids was found to be optimized one, from rest of ratios. The characterization confirmed the formation of liposomes. The entrapment efficiency was observed to be high as increasing the ratios between cholesterol and phospholipids, but then declined suddenly as further increasing the ratio. The best liposomal formulations showed the spherical shape with size ranging from <10µm. The optimized paclitaxel liposomes % release in phosphate buffer (pH 7.4) enhanced significantly, and rest of ratios. The optimized liposomal formulation also lowers the hemolytic toxicity of paclitaxel.