ISOLATION AND CHARACTERIZATION OF HEAVY METAL-REDUCING MICROBES FROM TANNERY EFFLUENT AND ITS ACTIVITY ON IMMOBILIZED BEADS
AbstractHeavy metal pollutants in tannery effluent can be removed using the bioremediation technique. This study intends to extract bacteria that may reduce heavy metals and molecularly characterize them using 16S rRNA PCR in samples taken from tannery effluent. It also studies their cell-free immobilized enzymatic activity. Thirteen bacterial isolates were identified based on morphological, cultural, and biochemical characteristics. The top four isolates, A, B, sp5, and sp3, were identified as Brevundimonas sp, Proteus mirabilis, and Alcaligenes faecalis by morphological, cultural, biochemical, MALDI-TOF Analysis, and molecular characteristics using 16S rRNA. These organisms showed good tolerance to zinc, nickel, and chromium. When heavy metals like zinc and chromium have reduction properties, by ICP-OES to assess the concentration of heavy metals. Further investigation, beads were immobilized, and its activity was analyzed.
Article Information
18
3392-3403
1879 KB
323
English
IJPSR
J. Raveena Jayam and C. Sivaraj *
ARMATS Biotek Training and Research Institute, Chennai, Tamil Nadu, India.
shivaraj27@gmail.com
24 October 2022
19 December 2022
31 December 2022
10.13040/IJPSR.0975-8232.14(7).3392-03
01 July 2023
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