ISOLATION AND CHARACTERIZATION OF POLYPHENOLIC COMPOUND QUERCITIN FROM PHYLLANTHUS EMBLICAAbstract
The curative parts of medicinal plants are not simply its woody stem or its leaves but the number of chemical compounds (phytoconstituents) it produces and uses for its own growth like flavonoids, alkaloids, gum, mucilage, protein, carbohydrate etc. The genus Phyllanthus is widely used in medicine but still lot of work left to explore this genus. So the species, Phyllanthus emblica which is widely used in Indian traditional system are selected for various screening. The mentioned plant materials are rich source of polyphenolic compounds and some typical type of polyphenolic are responsible for various pharmacological activities. The isolation and characterization of Phytoconstituents was done from the alcoholic extract by the chromatographic method. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences (IR, UV, 1HNMR, MS). A flavonoidal structure was isolated from the alcoholic extract of the plant. The yellow colored compound which solubilizes in methanol, ethanol and water having m.p. 315oC and Rf value 0.19 in mobile phase Isopropanol: formic acid: water (2:5:5) was identified by the various spectroscopical methods. So from this study it is concluded that isolated compound may be quercetin which is responsible for various pharmacological activities of the plants.
Prabodh Shukla*, Padmini Shukla and B. Gopalkrishna
EWS-915, Avas Vikas Colony-3, Kalyanpur Panki Road, Kanpur- 208017, Uttar Pradesh, India
20 January, 2012
23 February, 2012
23 April, 2012
01 May, 2012