ISOLATION AND PURIFICATION OF A GALACTOSE SPECIFIC LECTIN FROM SEEDS OF BAUHINIA VARIEGATA AND EVALUATION OF ITS ANTIMICROBIAL POTENTIAL
AbstractA galactose-specific lectin was purified from seeds of a leguminous plant, Bauhinia variegata, by affinity chromatography on lactose–agarose. Protein extract agglutinated human erythrocytes (treated with proteolytic enzyme). Among various carbohydrates tested, the lectin was best inhibited by D-galactose and other sacharides. SDS-PAGE showed that the lectin, named BVL produce a single band establishing that the lectin is composed of similar type of subunits. The antimicrobial activity of the purified lectin was carried out by agar ditch diffusion method at different concentrations using appropriate standards. BVL demonstrated a remarkable antibacterial activity against the pathogenic bacteria Pseudomonas aeroginosa, Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. BVL also shows a significant antifungal activity against Aspergilas niger and Penicilium crysogenum
Article Information
41
804-09
485
1678
English
IJPSR
Ravi Prakash Mishra*, Aijaz Ahmad Ganaie and Aashiq Hussain Allaie
Environmental Biotechnology Lab, Department of P.G Studies and Research in Bioscience, R.D. University, Jabalpur, Madhya Pradesh, India
sahilaejaz@gmail.com
05 May, 2015
18 January, 2016
23 January, 2016
10.13040/IJPSR.0975-8232.7(2).804-09
01 February, 2016
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