ISOLATION AND QUANTIFICATION OF GALLIC ACID FROM EULOPHIA OCHREATA LINDL. BY HPTLC

In the healthcare system, the development and modernization of phytomedicine in phytochemistry play a central and important role in drug development. Isolation and purification of bioactive compounds from naturally occurring substance are of prime importance. A sensitive and reliable high-performance thin-layer chromatography method has been developed for the estimation of gallic acid in the alcoholic extract of Eulophia ochreata Lindl. Alcoholic extract prepared was applied on silica gel G 60 F254 plate. The plate was developed using toluene: ethyl acetate: methanol: formic acid (6:6:0.4:1.6) as a mobile phase detection and quantification were performed by densitometric scanning at 275 nm. The system was found to give well-resolved bands for alcoholic extract having R_f values as 0.8 was matched with the standard R_f values like 0.82. The densitometric chromatogram of HPTLC fingerprint of the alcoholic extract, isolate, and standard gallic acid was obtained. The calibration curve of gallic acid was linear over a concentration range (0.2- 2 microg/ml) with a good correlation coefficient (R²= 0.9986) and coefficient of variation as CV- 2.4663%. The method was validated for linearity, precision, specificity, and it was found to be precise, reliable, and suitable. The proposed method is simple, rapid, precise, and accurate. The method was found to be suitable for qualitative and quantitative analysis of gallic acid in the alcoholic extract of Eulophia ochreata Lindl.