ISOLATION, CULTURE, AND ANTI-MRSA POTENTIAL OF A MARINE-ASSOCIATED BACTERIA ZOOSHIKELLA KANGWENSISAbstract
Isolation, identification, culturing, and chemical screening of a marine-associated bacterial strain, S6.2, were performed in this study. Anti-MRSA analysis of its extract was also conducted. Strain S6.2 was isolated from an unidentified red marine sponge and identified as Zooshikella kangwensis by 16S rDNA sequencing and biochemical characterization. On marine agar, the colonies embedded in the media and were pinkish and circular with oily smooth skin. Maximum growth was observed at 78 h (200 cfu/ml) and minimum growth at 168 h (23 cfu/ml).The purified bacteria were cultured in marine broth; cell density started to increase after 3 h and proceeded to the lag, exponential, stationary and dead phases at 24, 30, 78, and 132 h, respectively. Based on LCMS/MS analysis, strain S6.2 produced pinkish pigments: prodigiosin and two prodigiosin derivatives, namely 2-methyl-hexyl-prodiginine and 2-methyl-3-propyl-prodiginine. Anti-MRSA activity deduced by thin-layer chromatography (TLC) direct bioautography revealed that the active constituents contained in an ethyl acetate extract were nonpolar compounds. The MIC values varied between 12.5 and 25.0 µg/ml, while the MBC value was 50 µg/ml.
Rachow Khawchamnan, Monthon Lertcanawanichakul, Pharkphoom Panichayupakaranant, Wen Han Lin and Patchara Pedpradab *
Department of Marine Science, Faculty of Sciences and Fishery Technology, Rajamangala University of Technology Srivijaya, Sikao District, Trang Province, Thailand.
25 January 2021
05 May 2021
29 May 2021
01 December 2021