LARVICIDAL ACTIVITY OF LEAF EXTRACT OF GINGER AND TURMERIC ON MOSQUITO LARVAE
HTML Full TextLARVICIDAL ACTIVITY OF LEAF EXTRACT OF GINGER AND TURMERIC ON MOSQUITO LARVAE
Kalyani Khanra, Indranil Choudhuri and Nandan Bhattacharyya *
Department of Biotechnology, Panskura Banamali College, Panskura R. S., Purba Medinipur - 721152, West Bengal, India.
ABSTRACT: Dengue fever is an important public health problem in tropical and sub-tropical Continent. Recently the number of severe forms of the disease, dengue hemorrhagic fever, and dengue shock syndrome are increased. Dengue virus, a arbo-virus responsible for dengue fever and spread through its vector, Aedes aegypti. Controlling the mosquito is the good strategy to prevent this disease. The traditional chemical pesticides used to control mosquito have many drawbacks, and natural products are more acceptable in this aspect. The bio-efficacy of ginger (Zingiber officinale) and turmeric (Curcuma longa) ethanolic leaf extract was assessed against the fourth instars larvae of Aedes aegypti, under the laboratory conditions. Both shows moderate activity against Aedes aegypti larvae. However, the combined treatment of both leaf extract (1:1) shows highest activity. Combination of 50 µgml-1 turmeric and 50 µgml-1 ginger killed all of the population within 24 h. This is an eco-friendly and low-cost approach, may use to control Aedes aegypti.
Keywords: |
Ginger, Turmeric, Larvicidal activity, Aedes aegypti
INTRODUCTION: In tropical and sub-tropical continent including Asian country, risk of dengue fever is very high. Annually, there are 2 million infections, 500,000 cases of dengue hemorrhagic fever and 12,000 deaths 1. Aedes aegypti is generally known as a vector for an arbo-virus responsible for dengue fever, which is endemic to Southeast Asia, the Pacific island area, Africa and the America 2. This mosquito also acts as a vector of yellow fever in Central and South America and West Africa 3. However, dengue fever became an important public health problem as the number of reported cases continues to increase, especially with more severe forms of the disease, dengue hemorrhagic fever, and dengue shock syndrome, or with unusual manifestations like in central nervous system 4, 5.
A. aegypti is a cosmotropical species that proliferates in water containers in and around houses. Secondary vectors include Aedes albopictus, an important vector in Southeast Asia and that has spread to the America, Western Africa and the Mediterranean rim, Aedes mediovittatus in the Caribbean, Aedes polynesiensis and Aedes scutellaris in the western Pacific region. A. aegypti breeds in many types of household containers, like water storage tanks, flower vase etc 6.
Chemical insecticides used to control are harmful for human health as those contain neurotoxic and carcinogenic agents and are even carried through food chain which in turn affects the human through bioaccumulation. Moreover, due to repetitive use of same insecticide, vectors may become resistant 7, 8. Chemical insecticides are also toxic for non-target organisms like bees, butterfly etc., which have ecological importance. Therefore, alternative eco-friendly methods of controlling mosquito larvae are essential for controlling mosquito bearing diseases. Ginger (Zingiber officinale) and Turmeric (Curcuma longa) are use as spice in Indian subcontinent 9, 10. Different parts of both plants are traditionally use as medicine, and recent studies reveal diverse therapeutic properties 11, 12. India is the largest producer of ginger and turmeric and the rhizome of both plants have economical value. After harvesting, remaining part of the plants are going to waste and have no economic importance. In this study, we prepared the ethanolic extract of ginger and turmeric leaves and assessed the larvicidal activity against Aedes aegypti under laboratory conditions.
MATERIALS AND METHODS:
Collection of Plant and Preparation of Extract: Plants were collected from cultivation field near Panskura, West Bengal, India (22°24'01.6" N, 87°44'48.9" E). The voucher specimen kept in our research laboratory for further reference (NB/2015/21 and NB/2015/22). Plant leaves were cleaned thoroughly and shade dried at room temperature, crashed by an electrical blender to produce powder. The eathanolic extract of plant material was prepared at room temperature in 1:100 (w/v) ratio. The extracts were filtered through a Buchner funnel with Whatman number 1 filter paper and the filtrate was dried in air 13.
These metabolite extracts were then dissolved in ethanol to obtain a final concentration of 1000 mgl-1 as a stock plant extract.
Larval Toxicity Test: A laboratory reared colony of A. aegypti larvae was used for the larvicidal bioassay. The larvicidal activity was done as per guideline of World health organization 14 with modifications. In brief, 20 number of fourth instars mosquito larvae were placed in a conical flask with 249 ml phosphate buffered saline, maintain at room temperature with 12/12 h light dark cycle. The mosquito larvae were treated with different concentrations of plant extract from a 1000 mgl-1 stock. After 24 h and 48 h count the number death larvae and mortalities was calculated against an untreated control by the following formula 15.
% of Mortality = (Mortality in treatment/ Mortality in control) * 100
The experiment was performed in triplicate and result is expressed as average of three experiments.
RESULTS: Mortality of A. aegypti after the treatment of ginger and turmeric extract are shown in Table 1.
TABLE 1: RESULTS OF THE MORTALITY OF IV INSTAR A. AEGYPTI LARVAE AFTER THE TREATMENT OF PLANT EXTRACT AT DIFFERENT CONCENTRATIONS AND IN DIFFERENT PERIOD
Plant extract | Concertation (µgml-1) | % of Mortality (24 h) | % of Mortality (48 h) |
Turmeric | 25 µgml-1 | 10 | 22 |
50 µgml-1 | 25 | 35 | |
75 µgml-1 | 32 | 47 | |
100 µgml-1 | 38 | 61 | |
Ginger | 25 µgml-1 | 10 | 35 |
50 µgml-1 | 40 | 49 | |
75 µgml-1 | 38 | 75 | |
100 µgml-1 | 68 | 70 | |
Turmeric + Ginger (1:1) | 12.5 µgml-1 turmeric + 12.5 µgml-1 ginger | 20 | 75 |
25 µgml-1turmeric + 25 µgml-1 ginger | 45 | 100 | |
50 µgml-1 turmeric + 50 µgml-1 ginger | 100 | 100 | |
75 µgml-1 turmeric + 75 µgml-1 ginger | 100 | 100 |
FIG. 1: LARVICIDAL ACTIVITY OF GINGER AND TURMERIC LEAF EXTRACT. (A) MORTALITY AFTER 24 H TREATMENT (B) MORTALITY AFTER 48 H TREATMENT (C) COMBINE EFFECT OF BOTH AFTER 24 H AND 48 H
DISCUSSION: Mosquito life cycle follows eggs, larval stage, pupa and adult stage. The larval stage is more attractive target to kill them as it moves on water at this stage, which makes it easy to deal with them in this own habitat. Some fishes also take these larvae as their foods. The use of conventional chemical insecticides in the water sources, ultimately introduces many health risks to people and our ecosystem. Insecticides produced from plant, are more acceptable in this regard.
In this study, the mortality of larvae after 24 h treatment of ginger and turmeric leaf extract showed 38 % and 68 % respectively at a concentration of 100µgml-1 (Fig. 1). After 48 h, it was increased to 61 % and 70 % respectively. Hence, standalone use of both ginger and turmeric leaf extract not any significance. The combination of both leaf extract in 1:1 is able to kill all of the population after 24 h at 100 µgml-1 concentration.
This result clearly reveals that both the leaf extract of ginger and turmeric could serve as a potential larvicidal agents against the A. aegypti larvae and they have demonstrated a synergist act too.
CONCLUSION: The present strategy might be used to control dengue vector. This approach could reduce the possibilities of physiological resistance development in mosquito population. Before that, the mode of action and larvicidal efficiency of the both plants extract under the field conditions should be evaluated. Besides, further investigation is required regarding the effect on non-target organism, which is extremely important.
ACKNOWLEDGEMENT: Nil
CONFLICT OF INTEREST: The authors declare that they have no conflict of interest.
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How to cite this article:
Khanra K, Choudhuri I and Bhattacharyya N: Larvicidal activity of leaf extract of ginger and turmeric on mosquito larvae. Int J Pharm Sci Res 2018; 9(5): 2034-36.doi: 10.13040/IJPSR.0975-8232.9(5).2034-36.
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Article Information
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2034-2036
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English
IJPSR
K. Khanra, I. Choudhuri and N. Bhattacharyya*
Department of Biotechnology, Panskura Banamali College, Panskura R. S., Purba Medinipur, West Bengal, India.
bhattacharyya_nandan@rediffmail.com
01 August, 2017
10 October, 2017
20 October, 2017
10.13040/IJPSR.0975-8232.9(5).2034-36
01 May, 2018