METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND LOSARTAN IN TABLET DOSAGE FORM BY RP-HPLC
AbstractFixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple dosing regimens and their synergistic antihypertensive action, a combination of hydrochlorothiazide and losartan potassium is widely prescribed. In this study, an HPLC-UV method was developed and validated for the simultaneous determination of hydrochlorothiazide and losartan in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, ACE3-C18 column (250 mm × 4.6 mm 5 µm), 20 µl injection volume, the flow rate of 1 ml/min at ambient temperature (25 °C), and 226 nm. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 ≥ 0.997), and low LOD and LOQ (0.5 and 1.5 µg/ml, respectively). Successful application on pharmaceutical dosage tablet form gave recovery percent within acceptance criteria (92% and above) indicating that the proposed method is simple and reliable for the determination of LOP and HCTZ and hence can be applied for routine analysis in quality control laboratories.
Article Information
23
227-231
347
1140
English
IJPSR
N. M. S. Mohammed *, H. R. Abdo and H. M. Hassan
School of Chemistry, Faculty of Science, University of Zakho, Duhok, Kurdistan, Iraq.
nidhalsher@yahoo.com
08 May 2018
19 June 2018
13 July 2018
10.13040/IJPSR.0975-8232.10(1).227-31
01 January 2019
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