MOLECULAR DETECTION OF β-LACTAMASE GENE [SHV] IN KLEBSIELLA PNEUMONIAE

Klebsiella pneumoniae is a significant pathogen for nosocomial infections and quickly becomes the most prevalent beta-lactamases produced that are resistant to several antimicrobials. To comprehend deeper the condition of multidrug resistance [MDR]. Objectives: Our research was directed at identifying Klebsiella pneumoniae isolates containing shv β-lactamase gene and the relationship between them and some antimicrobial resistance in order to prevent rejection of therapy. Methods: Overall, 64 K. pneumonia it gathered pneumonia isolates and conducted a drug susceptibility test for 13 different antibiotics by the system Vitek 2 to identify their susceptibility patterns. Phenotypic tests for species producing ß-lactamase have determined the resistance phenotype of isolates. Results: The information indicated that 60[93.7%] of the entire isolates tested were Shv genes, while the remainder were positive. The PCR was used to amplified drug resistance. Highest antibiotic resistance in instances of ESBL positive. In fact, 2[33.3%] of the phenotypical confirmatory Test [PCT] sample were shown to be negative isolates. The data has shown that prediagnosed positive phénotype isolates were genetically positive for EsBL development. Concept: the collection of resistant strains is an alert in order to create rigorous controls on an infection that prevent b-lactamase genes from expanding. The surveillance of medicinal isolates and the reasonable use of antimicrobials therefore become important in order to reduce the propagation and incidence of the strength processes causing this measure.