MOLECULAR GENETICS STUDY ON HIGH AND INTERMEDIATE RISK GENOTYPES OF HUMAN PAPILLOMAVIRUS AMONG PATIENTS WITH BENIGN AND MALIGNANT CERVICAL LESIONS

Background and Objectives: It is well realized that cervical cancer is one of the most critical diseases in the world. This study has been laid down for molecular detection (qualitatively and quantitatively) and genotyping of high and intermediate risk HPV genotypes in patients with high suspicion of cervical cancer. Patients and Methods: A total of 120 females were included in this study. DNA has been extracted automatically using Samaga DNA extraction unit. Real time-PCR technique for detection and genotyping of high and intermediate risk genotypes was performed. Results: The genes amplification revealed that only 9(11.3%) out of 80 were positive for high-risk HPV. The genotyping study yielded that the most prevalent genotypes were HPV 16, 18, 39, 33 and 35. Among the result of high-risk genotypes of HPV, the viral load was 6.42 ± 0.71 for clinical status CIN III. Only 11 out of 20(55 %) of FFPE were positive for high-risk HPV. On the other hand, The clinical status of high-risk HPV genotypes from FFPE samples were 4(20%), and 5(25%) for A7 and A9 with clinical status CIN II and viral load was 5.57 ± 0.75 for A7 and 6.28 ± 0.75 for A9. Conclusion: The study concluded that the use of Real-Time PCR as a diagnostic and genotyping tool for HPV has the advantage for women who currently have high-grade cervical lesions and at high risk of developing cervical cancer in the future.