NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF EMPAGLIFLOZIN IN HUMAN PLASMAAbstract
A new reversed phase simple, economic and specific validated high performance liquid chromatography method for the estimation of empagliflozin in human plasma is presented. A chromatographic separation was carried out isocratically by mobile phase comprising of methanol and acetonitrile, 50:50% v/v at a flow rate of 1.0 ml/min on agilent long C18 column (250 mm × 4.6 mm, 5 µm) with photodiode arrays (PDA) detection at 270 nm. The bioanalytical procedure involves deproteination of plasma with 30% ethyl acetate and liquid-liquid extraction process. Chromatogram showed a peak of empagliflozin at retention time of 8.898 min. The correlation coefficient (r2) was found to be 0.999 in the concentration range of 50-150 μg/ml. No interference peak was observed in blank plasma samples at the retention time of empagliflozin. The percentage of relative recovery and coefficient of variation (CV %) values of precision and accuracy were within the acceptable limits. The method proved simple, cost effective and sensitive for estimation of empagliflozin in human plasma.
N. Padmaja, T. Desalegn, M. Sharathbabu and G. Veerabhadram *
Department of Chemistry, University College of Science, Osmaina University, Hyderabad, Telangana, India.
07 March, 2018
16 May, 2018
31 May, 2018
01 November, 2018