NOVEL ISOCRATIC RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF OFLOXACIN AND BECLOMETHASONE DIPROPIONATE

The present paper describes a novel, isocratic, simple, precise, accurate, and robust reversed-phase high-performance liquid chromatographic (RP-HPLC) method development and validation for simultaneous quantitative estimation of Ofloxacin and Beclomethasone dipropionate in an Ophthalmic/Otic formulation. The proposed chromatographic estimation was carried out isocratically using Protonsil C18 (250 × 4.6mm) SH 5.0µm column, the mixture of 0.02 M potassium dihydrogen phosphate buffer (pH adjusted to 3 using ortho-phosphoric acid): acetonitrile in a ratio of 30:70 v/v with a flow rate of 1 mL/min was used as mobile phase and column oven adjusted to 30°Cwith injection volume 10µL. The ultraviolet (UV) detection was carried out at 234 nm. The retention time of ofloxacin and beclomethasone dipropionate was found to be 2.67±0.2 min and 7.42±0.2 min, respectively. Calibration curves were linear over the tested concentration range of 4 to 20µg/mL. The present study reveals that this novel isocratic HPLC method is well-validated, reliable, and can be used for routine analysis of ofloxacin and beclomethasone dipropionate in an otic formulation containing these as one of the ingredients.