NOVEL RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF RESERPINE AND PIPERINE IN POLYHERBAL FORMULATION
AbstractA novel, simple, accurate, precise, robust High-Performance Liquid Chromatography method was developed to simultaneously estimate reserpine and piperine. This method was applied to standardize polyherbal formulation containing these markers as one of its ingredients. The markers were resolved using Prontosil C-18 column, with mobile phase acetonitrile and water containing 0.05% ortho-phosphoric acid in the ratio 50:50, flow rate of 1 ml/min and detector set at 225 nm. Retention time of reserpine and piperine were found to be 5.4±0.2 min and 13.5 ± 0.2 min, respectively. Linear response was obtained in the tested concentration range of 1 to 20 µg/ml for reserpine and 10 to 100 µg/ml for piperine, with correlation coefficients of 0.999 and 0.998 for reserpine and piperine, respectively. This method can be used to evaluate other formulations containing reserpine and piperine as markers, thus conforming to the need of ensuring safety and quality of herbal formulations.
Article Information
40
3544-3549
609 KB
326
English
IJPSR
U. D. Jaiswar, R. M. Patil and V. N. Jain *
Department of Quality Assurance, Oriental College of Pharmacy, Sector 2, Sanpada, Navi Mumbai, Maharashtra, India.
vandana.jain@ocp.edu.in
03 November 2022
09 January 2023
01 May 2023
10.13040/IJPSR.0975-8232.14(7).3544-49
01 July 2023
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