NOVEL SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ACECLOFENAC AND CURCUMIN IN DEVELOPED DOSAGE FORM

Introduction: Aceclofenac (ACE) and Curcumin (CUR) are anti-inflammatory drugs utilized for osteoarthritis and rheumatoid arthritis. ACE, As NSAID inhibits prostaglandins, while CUR, a natural polyphenol, exhibits antioxidant and anticancer properties. Combining them may offer synergistic benefits for inflammatory diseases. Objective: To develop a UV spectrophotometric method for simultaneous determination of ACE and CUR from a developed dosage form, focusing on simplicity, Accuracy and cost-effectiveness. The method employs absorbance correction to calculate drug concentrations based on their absorbance difference at two wavelengths. Method: Using a solvent of phosphate buffer pH 5.5: ethanol (1:1), standard solutions of ACE (5-30 µg/mL) and CUR (1-7 µg/mL) were prepared. UV spectrophotometer measured absorbance at 276 nm and 430 nm for both standard and sample solutions. Absorbance correction equations were used for concentration determination. Result: Method validation followed ICH guidelines, demonstrating good linearity (correlation coefficients: 0.999 for ACE, 0.998 for CUR), accuracy (recovery values: 92.72% for ACE, For cur) and precision (RSD < 2% for intra-day and inter-day variations). Specificity was confirmed, and method robustness was evaluated by varying buffer pH, solvent ratio, and wavelength, showing no significant impact. Limits of detection (LOD) and quantification (LOQ) were 0.16 µg/mL and 0.49 µg/mL for ACE, and 0.08 µg/mL and 0.25 µg/mL for CUR. Conclusion: The proposed UV spectrophotometric method offers a simple, accurate, and cost-effective approach for the simultaneous determination of ACE and CUR from a developed dosage form, suitable for routine analysis in quality control laboratories.