OLIBANUM RESIN BASED AMBROXOL HYDROCHLORIDE TABLETS: FABRICATION AND IN VITRO EVALUATION
HTML Full TextOLIBANUM RESIN BASED AMBROXOL HYDROCHLORIDE TABLETS: FABRICATION AND IN VITRO EVALUATION
Y. Indira Muzib*1, Padma Sree. Kurri 1 and Y.R. Ambedkar 2
Institute of Pharmaceutical Technology, Sri Padmavathi Mahila University, Tirupathi – 517502, Andhra Pradesh, India
Govt. Veterniary Poly. College, Gariveedu, Vizayanagaram, Andhra Pradesh, India
ABSTRACT: Present era meant for utilization of natural resources in the development of dosage forms. In the present investigation natural biodegradable polymer olibanum resin is employed to develop matrix tablets of ambroxol hydrochloride for sustained release. Various Ratios of resins is study its ability to retard the early release of the drug from formulation. The tablets were prepared by wet granulation technique and were subjected for their thickness, weight variation test, drug content, hardness and friability. The in vitro dissolution studies were carried out using USP apparatus type II (paddle) at 100 rpm. Drug content of the formulations ranged between 97.78±0.08 to 100.12, where as hardness varies from 5.9-7.6kg/cm2. From the in vitro release studies it was found that F5 formulation was similar to marketed sample hence it was selected as a optimized formulation. Higuchi release kinetics shows r2=0.9298, from Korsemeyer- Peppas plot n value >0.89 indicates super case II type of release indicating that the drug release from these formulations is by erosion of polymer. The optimized formulation of Ambroxol hydrochloride matrix tablets (10%w/w) were subjected to short term stability studies at 45°C with 75% RH for 45 days revealed that no significant differences in physical appearance, drug content, dissolution, T50 and T90 were found
Keywords: |
Ambroxol hydrochloride,
matrix tablets, olibanum resin,
In vitro drug release
INTRODUCTION: Nowadays researchers focused on utility of natural and biodegradable polymers, to minimize usage of synthetic materials in the preparation of pharmaceutical dosage forms. Nature provides us lot of excipients like waxes, and gums etc., Guargum 1, Olibanum gum 2, gum karaya 3, xanthan gum 4, chitosan 5, pectin 6 , resin 7 etc. in the development of controlled and sustained release formulations , colon targeted drug delivery systems8, 9 for a variety of therapeutic agents
Drug embedded in an insoluble matrix provides a convenient means of controlling the drug release. In this type of system, drug release is followed by penetration of dissolution medium in to the porous matrix to dissolve the drug, followed by diffusion/ leaching of the dissolved molecules out of the matrix. Previous studies shows that Olibanum [Frankincense] is an aromatic resin obtained from trees of the genus Boswellia, particularly Boswellia sacra Syn. B.carteri, B.thurifera [Burseraceae] used in aromatherapy. Silver and Hojari are generally considered the highest grades of frankincense.
In ayurvedic medicine Indian frankincense [Boswellia serrata] has been used for hundreds of years for treating arthritis, healing wounds, strengthening the female hormone system 10.
Ambroxol hydrochloride has secretolytic effects. It stimulates serous cells of bronchi mucous membranes, increases mucous secretion content by changing the structure of bronchial secretion. It improves breathing and suppresses cough to some extent. A short biological half-life of 4hrs 11 calls for a frequent daily dose of 30mg 3-4 times a day 12. The aim of the present study was to design sustained release dosage form of Ambroxol to prolong clinical efficacy, minimize side effects and to reduce dosing 13.
Few works has been done on natural polymer like gum olibanum and resin 14, 15, 16 there is no evidence that polymers like olibanum resin was used for formulating sustained release matrix tablets of Ambroxol hydrochloride. Hence trials were made to study the utility of olibanum resin in retarding the drug release for 12 hrs.
MATERIALS AND METHODS:
Ambroxol hydrochloride was a gift sample from Sree Sai Organics Pvt. Ltd., Kondapalli, Vijayawada. Gum Olibanum was obtained from Girijan co-operative corporation Ltd, Visakhapatnam. All other chemicals used were of analytical grade, and procured from commercial sources.
Analytical Method of Ambroxol Hydrochloride:
In this investigation, UV Spectrophotometric estimation based on the measurement of extinction at 248 nm in pH 1.2 and pH 6.8 phosphate buffers were used for the estimation of Ambroxol hydrochloride. 17
Isolation of olibanum resin from gum olibanum:
Powdered olibanum [10gm] was extracted repeatedly with 200ml quantity of solvent ether. The ether extracts were collected in a porcelain dish and concentrated to dry at 40°C to obtain resin fraction. The dried mass was powdered and size was reduced to 200 # mesh.
Preparation of matrix tablets 18
Different tablet formulations were prepared by wet granulation technique Table 1. Accurately weighed quantities of pre-sieved drug and matrix material were mixed uniformly and wetted with isopropyl alcohol: water [1:1] as granulating fluid, the cohesive mass thus obtained was screened through a sieve no 16. The granules were dried at 50°C in hot air oven till constant weight was reached. The coarse granules so obtained were once again screened using the same sieve. Talc and magnesium stearate were finally added as glidant and lubricant and were compressed to tablets. Prior to compression, the granules were evaluated for various IPQC tests.
TABLE 1: COMPOSITION AMBROXOL HYDROCHLORIDE MATRIX TABLET
Batch No | Drug(mg) | Olibanum Resin | Avicel pH 101 | Starch | Talc | Mg stearate | Total weight(mg) |
F1 | 75 | 75 | - | 7.5 | 3 | 1.5 | 162 |
F2 | 75 | 112.5 | - | 9.5 | 4 | 2 | 203 |
F3 | 75 | 150 | - | 11.5 | 5 | 2.5 | 244 |
F4 | 75 | 8.5 | 72.9 | 8.5 | 3.4 | 1.7 | 170 |
F5 | 75 | 17 | 64.4 | 8.5 | 3.4 | 1.7 | 170 |
F6 | 75 | 25.5 | 55.9 | 8.5 | 3.4 | 1.7 | 170 |
*Quantity (mg) present per each Matrix tablet
Evaluation of granules:
For all the formulations the prepared granules were evaluated for flow properties like angle of repose, loose bulk density, tapped bulk density, compressibility index and Hauser ratio.
Angle of repose θ = tan-1 [h/r]
Loose bulk density = Weight of powder/Volume of packing.
Tapped bulk density= Weight of powder/tapped volume of the packing.
Compressibility Index = 100 × Tapped density / Bulk density
Hauser ratio = Tapped density/ Bulk density
Drug content was determined by extracting powdered granules [75mg] of Ambroxol hydrochloride with water and for the filtered [0.45-μ membrane] solution absorbance was measured at 248nm. Loss on drying and Moisture content were determined by placing 1gm of granules in an oven which was maintained at 105°C and dried up to constant weight and were calculated from the formula.
Loss on drying = Initial weight - Final weight/Initial weight x 100
Moisture content = Initial weight - Final weight/Final weight x 100
Physicochemical Compatibility of Drug and Polymer:
The infrared (IR) spectra were recorded using an FTIR spectrophotometer (Bruker FTIR) by the ATR method in the wavelength region between 4000 and 400cm–1 for pure drug Ambroxol hydrochloride and polymer olibanum resin. The spectra obtained for drug, polymer and formulation were compared.
Physico-chemical characterization of tablets:
The prepared matrix tablets were evaluated for hardness, weight variation, thickness, friability and drug content. Tablet hardness was determined for 5 tablets using a Monsanto tablet hardness tester. Friability was determined by taking 10 tablets in Roche friabilator. The weight variation was evaluated on 20 tablets using an electronic balance, and the test was performed according to the official method. For determining the drug content, three tablets from each formulation were crushed and powder containing 75mg of Ambroxol hydrochloride was dissolved in 75ml of phosphate buffer pH 6.8. From this 10µg/ml, equivalent solutions were prepared and drug content of Ambroxol hydrochloride was determined by measuring the absorbance of samples at 248nm using UV/Visible spectrophotometer. The swelling behavior of the optimized formulation [F5] was studied. The % increase in weight due to absorbed liquid or water uptake was estimated at each time point. The physicochemical properties of designed tablets are shown in Table 3.
In vitro drug release &kinetic studies 19, 20, 21
The in vitro dissolution studies were carried out using USP apparatus type II [paddle] in simulated gastric fluid [pH 1.2±0.1] for the first 2hrs and in phosphate buffer pH 6.8 from 3 to 12hrs. Rotation speed of 100rpm at temperature of 37± 0.2°C and dissolution medium of 900ml was maintained throughout the experiment. At predetermined time intervals, 5ml of samples were withdrawn and fresh dissolution medium was replaced after each withdrawal. The samples withdrawn were filtered through 0.45µ membrane filter, and absorbance was measured spectrophotometrically at 248nm using a UV-visible spectrophotometer and cumulative percent drug release was calculated. All dissolution studies were carried out in triplicate.
The same was carried out on commercial Ambroxol SR capsules for comparative evaluation. The rate and mechanism of release of Ambroxol hydrochloride from the prepared matrix tablets were analyzed by fitting the release data in to Zero-order, First order, Higuchi’s equation and Korsmeyer model. The value of n is <0.45 for fickian release, > 0.45 and < 0.89 for non-fickian release, 0.89 for the case II release and > 0.89 for super case II type release.
CALCULATION OF SIMILARITY FACTOR [F2] 22
Similarity factor was calculated for the optimized formulation [F5] with the marketed sample using the formula f2 = 50 + log {[1+ [1/n] ∑t=1 * n [Rt-Tt]2]-0.5 *100} where n is the number of dissolution sampling times, Rt and Tt are the individual or mean percent dissolved at each time point for the reference and test dissolution profiles respectively.
Stability Studies:
Stability studies were conducted on Ambroxol hydrochloride matrix tablets containing 10% W/W olibanum resin to assess their stability with respect to their physical appearance, drug content and drug release characteristics after storing them at 45°C with 75% RH for 45 days.
RESULTS AND DISCUSSION:
FTIR studies:
IR spectra of the pure drug and the optimized formulation F5 was compared and no interaction was observed between the drug and polymer used for the study as there is no shift of positions of the bands in both the spectra shown in Fig.1, 2, 3. The IR spectrum shows that both drug and polymer were not interacted each other and appear as separate entities which is clearly shown in the spectra. Both hydroxyl and secondary amino group stretching vibration were merged to each other and therefore appear on single strong broad band at 3393.82 cm-1 for Ambroxol pure drug. In case of Ambroxol and secondary amine of Ambroxol vibrations were merged to each other and appear as a single broad hydrogen bonded band for F5 the peak was at 3391.93 cm-1.
FIGURE .1 FTIR SPECTRA OF AMBROXOL HYDROCHLORIDE
FIGURE .2 FTIR SPECTRA OF OLIBANUM RESIN
FIG.3: FT IR SPECTRA OF F5MATRIX TABLETS
Physical properties of granules:
The prepared granules were evaluated for angle of repose, loose bulk density (LBD), tapped bulk density (TBD), compressibility index (CI) and Hausner ratio given in Table 2. The angle of repose of all the formulations granules was found to be in the range of 25-30° for which the flow was found to be excellent and except F1 shows the flow was good. Based on the CI, HR for the formulations F3, F4, F5 and F6 the flow was good and for F1, F2 the flow property was fair.
TABLE 2: PHYSICAL PROPERTIES OF GRANULES FORMULATED WITH OLIBANUM RESIN*
Batch No | Angle of Repose | LBD (g/ml) | TBD (g/ml) | CI (%) | HR | DC (%) | MC (%) | LOD (%) |
F1 | 30.16±0.07 | 0.33±0.02 | 0.41±0.02 | 19.51±0.03 | 1.24±0.02 | 99.61±0.02 | 4.9±0.2 | 4.5±0.3 |
F2 | 27.67±0.05 | 0.33±0.03 | 0.39±0.04 | 15.38±0.01 | 1.18±0.03 | 97.78±0.03 | 4.4±0.7 | 3.9±0.5 |
F3 | 25.85±0.04 | 0.33±0.08 | 0.37±0.04 | 10.81±0.05 | 1.12±0.05 | 98.61±0.01 | 3.8±0.5 | 3.3±0.1 |
F4 | 27.11±0.06 | 0.38±0.05 | 0.43±0.07 | 11.62±0.01 | 1.13±0.03 | 100.24±0.06 | 4.8±0.4 | 4.3±0.4 |
F5 | 25.98±0.04 | 0.41±0.03 | 0.47±0.02 | 12.76±0.05 | 1.14±0.04 | 99.87±0.03 | 4.5±0.4 | 4.0±0.3 |
F6 | 26.35±0.02 | 0.45±0.08 | 0.51±0.05 | 11.76±0.04 | 1.13±0.06 | 97.42±0.08 | 4.1±0.3 | 3.7±0.2 |
* All the values are expressed as mean± SD, n=5
Physical properties of tablets:
The compressed tablets were evaluated for physical properties like weight variation, hardness, friability and content of active ingredient. The results obtained were within the prescribed limits. The results are tabulated in the Table 3.
TABLE 3: PHYSICAL PROPERTIES OF TABLETS FORMULATED WITH OLIBANUM RESIN*
Batch No | Wt in mg | Hardness (Kg/cm2) | Thickness (mm) | Friability (%) | DC (%) |
F1 | 160.5±0.09 | 5.9±0.7 | 2.81±0.05 | 0.67±0.02 | 98.04±0.03 |
F2 | 203.8±0.03 | 6.1±0.3 | 3.49±0.03 | 0.36±0.07 | 99.12±0.02 |
F3 | 242.6±0.08 | 6.5±0.5 | 3.91±0.08 | 0.59±0.09 | 97.78±0.08 |
F4 | 171. 3±0.03 | 6.8±0.2 | 2.88±0.02 | 0.22±0.04 | 98.90±0.04 |
F5 | 168.5±0.07 | 7.1±0.8 | 2.92±0.03 | 0.48±0.04 | 99.83±0.06 |
F6 | 169.3±0.02 | 7.6±0.4 | 2.94±0.07 | 0.31±0.05 | 100.12±0.02 |
*All the values are expressed as mean ±SD, n=5
In vitro release studies:
FIG. 4: DISSOLUTION PROFILES OF AMBROXOL HYDROCHLORIDE MATRIX TABLETS FORMULATED WITH OLIBANUM RESIN
FIG. 5: ZERO ORDER PLOT OF AMBROXOL HYDROCHLORIDE MATRIX TABLETS FORMULATED WITH OLIBANUM RESIN
FIG. 6: HIGUCHI PLOT OF AMBROXOL HYDROCHLORIDE MATRIX TABLETS FORMULATED WITH OLIBANUM RESIN
With olibanum resin firstly 1:1, 1:1.5, 1:2 ratio’s [F1, F2, F3] were formulated but even at the 12th hour the release was too low. So, a 5%, 10% and 15% resin containing formulations were prepared and conducted drug release studies. 5%, 10% resin containing formulations (F4, F5) failed to sustain the drug release till 12th hour but F5 gave a close release profile with that of MS so F5 was selected as optimized formulation. F6 with 15% resin showed 99.86% drug release at 12th hour. The release data of matrix tablets were fitted into various mathematical models to evaluate the kinetics and mechanism of drug release from the tablets (Fig. 4, 5, 6). The model that best fits the release data is selected based on the correlation coefficient (r2) value in various models. The model that gives high “r2” value is considered as the best fit of the release data. The “r2” values for zero order, first order, Higuchi model and Korsmeyers-Peppas plot are given in the Table 4.
These results indicate that the drug release from the formulations F4, F5, F6 followed Zero order kinetics whereas, the formulations F1, F2, F3 followed Higuchi kinetics. Release of the drug from a matrix tablet containing hydrophilic polymers generally involves factors of diffusion. To evaluate drug release mechanism from the tablets, plots of percent released versus square root of time as per Higuchi’s equation were constructed. The formulations F1, F2, F3 show better linearity for Higuchi release kinetics with (r2>0.99). It indicates that the drug release is by diffusion mechanism.
TABLE 4: RELEASE KINETICS OF AMBROXOL HYDROCHLORIDE MATRIX TABLET FORMULATED WITH OLIBANUM RESIN
Batch No | Release Model | T50(hrs) | T90(hrs) | |||||||
Zero order | First order | Higuchi | Koresmeyer- | Peppas | ||||||
r2 | k | r2 | r2 | k | n | r2 | k | |||
F1 | 0.7967 | 6.4873 | 0.9295 | 0.9914 | 19.1424 | 0.5286 | 0.9844 | 18.4017 | 6.9 | 16.8 |
F2 | 0.9143 | 5.0529 | 0.9708 | 0.9957 | 14.6778 | 0.5888 | 0.9971 | 12.3660 | 11.1 | 20.6 |
F3 | 0.9172 | 4.4402 | 0.9669 | 0.9944 | 12.8861 | 0.5734 | 0.9961 | 11.1574 | 12.6 | 22.8 |
F4 | 0.9938 | 13.1994 | 0.7628 | 0.9044 | 27.8107 | 0.9138 | 0.9880 | 14.4074 | 4.1 | 6.3 |
F5 | 0.9993 | 9.9164 | 0.7870 | 0.9298 | 25.2422 | 0.9484 | 0.9982 | 10.8391 | 4.8 | 9.2 |
F6 | 0.9903 | 8.2601 | 0.7277 | 0.9441 | 23.2657 | 0.7909 | 0.9953 | 12.7537 | 6.3 | 10.7 |
The dissolution data was fitted to Korsmeyer equation which is used to describe the drug release behaviour from polymeric systems. The formulations F1, F2, F3 showed diffusion co-efficient value (n) greater than 0.45 but less than 0.89 shown in the Table 4. So, it indicates Non-Fickian [anomalous] transport mechanism indicating that the drug release is by a combination of both diffusion and erosion of polymer. And the formulations F4, F5, F6 showed n value >0.89 indicates super case II type of release indicating that the drug release from these formulations is by erosion of polymer.
Similarity factor was calculated for the formulations, it was found that F5 formulation is to the release profile of marketed sample showed in the Table 5, hence it was optimized and subjected for further studies.
Swelling index:
The swelling index of the optimized formulation F5 was found to be 59.12%. Plastic viscosity was found to be 0.21centipoise. It was observed that there is an inverse relation for swelling index and plastic viscosity with release rate constant.
Stability Studies:
The optimized Ambroxol hydrochloride matrix tablets F5 were packed in amber colored screw capped bottles and subjected to short term stability studies at 45°C with 75% RH for 45days as per ICH guidelines showed negligible change over time for the parameters like appearance, the drug release profiles, drug content, T50 and T90 (Table 5).
TABLE 5: SIMILARITY FACTOR OF AMBROXOL HYDROCHLORIDE MATRIX TABLETS FORMULATED WITH OLIBANUM RESIN (F5) AND MS (f2=68.96)
S.no | Time | Average % Drug release | f2 | MDT(T) / MDT(R) | AUC(T) / AUC(R) | |
Reference | Test | |||||
1 | 0 | 0.000 | 0.000 | 0.000 | 0.000 | 0.000 |
2 | 0.5 | 6.25 | 6.25 | 100.00 | 1.000 | 1.000 |
3 | 1 | 13.59 | 10.67 | 85.38 | 0.879 | 0.888 |
4 | 1.5 | 18.72 | 15.06 | 79.69 | 0.955 | 0.837 |
5 | 2 | 22.69 | 19.16 | 77.57 | 1.017 | 0.833 |
6 | 3 | 33.35 | 28.70 | 74.64 | 1.022 | 0.844 |
7 | 4 | 39.79 | 40.07 | 76.12 | 1.158 | 0.883 |
8 | 5 | 56.02 | 52.08 | 75.05 | 1.020 | 0.911 |
9 | 6 | 58.78 | 61.18 | 75.44 | 1.128 | 0.933 |
10 | 7 | 76.42 | 69.97 | 72.23 | 0.972 | 0.943 |
11 | 8 | 79.39 | 77.28 | 72.82 | 1.036 | 0.943 |
12 | 9 | 85.35 | 87.87 | 73.19 | 1.089 | 0.955 |
13 | 10 | 90.35 | 99.51 | 68.96 | 1.150 | 0.974 |
CONCLUSION: The present study was to make an evaluation of sustained release matrix tablets of Ambroxol hydrochloride formulated with olibanum resin as a release retardent and to conduct the drug release kinetics to assess the drug release mechanism. From the studies it was concluded that olibanum resin successfully control the early release of drug and extends the release till 12 hours. Release of drug was followed diffusion and erosion mechanism. The %W/W of polymer plays a major role in overall release of the drug and the formulated matrix tablets with 10% olibanum resin offered comparative release profile with that of marketed sample.
ACKNOWLEDGEMENTS: Authors thank to Sri Padmavathi Mahila University, Tirupati and Bapatla College of pharmacy, Bapatla for providing facilities to carry this project work.
REFERENCES:
- Bharathi A, Sushma Ch, Silpika K, Deepthi KNV and Bhagya Lakshmi S: Formulation Development and Evaluation of Sustained Release Matrix Tablets of Quetiapine Fumarate Journal of Chemical and Pharmaceutical Research 2014; 6(4): 628-632.
- Chowdary KPR, Udaya Chandra D: Design and Evaluation of Floating Tablets of Pioglitazone Employing Selected Natural and Synthetic Polymers. International Research Journal of Pharmaceutical and Applied Sciences 2014. 2014; 4(1): 94-99.
- Raju B, Uma Maheshwar Rao V, Sujitha H, Saikrishna K, Venkateshwarlu Ch and Leela Keerthi: Formulation and In Vitro Evaluation of Mucoadhesive Buccal Tablets of Fluvastatin. An International Journal of Advances in Pharmaceutical Sciences 2014; 5(6): 2499-2510.
- Rajat K, Snehamayee M, Satyabrata B, Debjyoti Das and Bhaktibhusan B: Formulation and In Vitro Characterization of Xanthan Gum-Based Sustained Release Matrix Tables of Isosorbide-5- Mononitrate. Iranian Journal of Pharmaceutical Research 2010 Winter; 9(1): 13–19.[PMCID: PMC3869548]
- Jain H, Patel K, Kanzariya V, Shah H and Upadhyay U: Formulation and Evaluataion of Colon Targeted Matrix Tablet of Naproxen. International Journal of Pharmacy and Pharmaceutical Sciences 2014; 6(8): 183-186.
- Kishor B, Munira M and Hemant D; Optimisation and In Vivo Evaluation of Pectin Based Drug Delivery System Containing Curcumin for Colon. International Journal Of Biomaterials 2014; 924278: 1-7.
- Chowdary KPR and Rami Reddy G: Formulation and Evaluation of Diclofenac Controlled Release Tablets Employing Olibanum International Journal of Pharmaceutical Sciences and Research 2012 3(4): 1090-1095.
- Olibanum resin, willy Benecke, http://www.wilkeycom/olibanum resin_f.htm Accessed on: 27-08-2010.
- Barar FSK: Essentials of Pharmacotherapeutics, S Chand and Company Ltd New Delhi, Third Edition, 2005.
- Sweetman C and Martindale: The Extra The Pharmaceutical Press London, Thirty Three Edition 2002, 1084.
- Osteorhuis B, Storm G, Cornelissen PJ, Su CA, Sollie FA and Jonkman JH. Dose Dependant Effect of Ambroxol European Journal of Clinical Pharmacology 1993; 44: 237‐241.
- Alighieri T, Avanessian S, Berlini S, Bianchi SG, Deluigi P, Valducci R and Guelen PJ.. Bioavailability of ambroxol sustained release preparations. Part 1: Dissolution studies. Arzneim. Forsch‐ Drug Research 1988; 38: 92.
- Chowdary KPR, Mohapatra P and Murali Krishna MN: Evaluation of olibanum resin as microencapsulating agent for controlled drug delivery. Indian Journal of Pharmaceutical Science 200668(4): 461-464.
- Satyajit Panda: Formulation and evaluation of zidovudine loaded olibanum resin microcapsules: exploring the use of natural resins as biodegradable polymeric materials for controlled release. Asian Journal of Pharmaceutical and clinical Research 2013; 6(3).
- Indian Pharmacopoeia Ministry of Health and Family Welfare. Delhi: Controller of publication; 1985.
- Lachman L, Lieberman HA, Kanig JL. Eds., In: The Theory and Practice of Industrial Pharmacy. Varghese Publishing House Mumbai, Third Edition.
- Higuchi T: Mechanism of sustained-action medication: theoretical analysis of rate of release of solid drug dispersed in solid matrices. Journal of Pharmaceutical Sciences 1963; 52:1145–1149.
- Korsmeyer RW, Gurny R and Peppas N: Mechanisms of solute release from porous hydrophilic polymers. International Journal of Pharmaceutics 1983; 24: 25–35.
- Peppas NA. Analysis of Fickian and non-Fickian drug release from polymers. Pharmaceutica Acta Helvetiae 1985; 60: 110–111.
- Costa P and Jose MSL: Modeling and comparison of dissolution profiles, European Journal of Pharmaceutical Sciences 2001; 13:
How to cite this article:
Muzib YI, Kurri PS and Ambedkar YR: Olibanum Resin Based Ambroxol Hydrochloride Tablets: Fabrication and In Vitro Evaluation. Int J Pharm Sci Res 2015; 6(7): 2968-75.doi: 10.13040/IJPSR.0975-8232.6(7).2968-75.
All © 2013 are reserved by International Journal of Pharmaceutical Sciences and Research. This Journal licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
Article Information
39
2968-75
615
1205
English
Ijpsr
Y. Indira Muzib*, Padma Sree. Kurri and Y.R. Ambedkar
Institute of Pharmaceutical Technology, Sri Padmavathi Mahila University, Tirupathi, Andhra Pradesh, India
yindira1415@gmail.com
17 November, 2014
19 February, 2015
27 March, 2015
10.13040/IJPSR.0975-8232.6(7).2968-75
01 July, 2015