OPTIMIZATION AND DEVELOPMENT OF BIO SYNTHETIC SILVER NANO PARTICLES OF AZURIN- AN ANTI-CANCER AGENT FROM PSEUDOMONAS AERUGINOSA

The pretentious extract of Pseudomonas aeruginosa has shown promising anticancer activity. It contains a protein called Azurin. It has a molecular weight of 14 kDa. It is soluble in water. It is a type I copper-containing protein of cupredoxin family. In this work, Pseudomonas aeruginosa MTCC strain 647 was cultivated in modified Asparagine -proline broth. An attempt was done to isolate and purify azurin from Pseudomonas aeruginosa (MTCC 647) by using chromatography on sephadex G, CM cellulose and SDS PAGE electrophoresis. Recent studies have found that Pseudomonas aeruginosa can synthesize nano particles through either intracellular or extracellular mechanisms by bio synthetic means. Hence, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. P. aeruginosa MTCC strains 647 was cultivated in modified Asparagine -proline broth initially to produce shake flask cultures and then fed batch cultivation. From the bacterial extracted Azurin is isolated and purified to get a final concentration of 4.6 mg/g dry bacteria. As the nano particle mediated drug delivery effectively deliver the drug to the tumor cells, the composite nano particles (Azurin-Ag NP) were synthesized by biosynthetic methods. These bio synthetically produced Ag nano particles has loaded maximum 36 ng of Azurin protein. Pseudomonas aeruginosa MTCC strains 647 can be chosen as strain to produce Azurin in large scale. The anti-cancer activity of Azurin is complimented with bio synthetic production of silver nano particles containing Azurin (Azurin-Ag-NPs).