OPTIMIZED FORMULATION OF VANCOMYCIN LOADED THERMOREVERSIBLE HYDROGEL FOR TREATMENT OF ORTHOPEDIC INFECTIONSAbstract
The aim of the present study was to develop suitable controlled release injectable vancomycin thermoreversible chitosan/ β-glycerophosphate hydrogel system for treatment of orthopedic infections. The effect of different formulation variables on the gelation temperature was studied. The prepared vancomycin loaded Chitosan/β-glycerophosphate thermoreversible hydrogels were characterized regarding gelation temperature, in-vitro vancomycin release and enzymatic degradation rate in presence of lysozyme. Antimicrobial activity of the released vancomycin was tested against the gram-positive methicillin resistant Staphylococcus aureus (MRSA) by agar diffusion method. The results showed that chitosan/ β-glycerophosphate solution were able to form gels at body temperature depending on chitosan degree of deacetylation, concentration, pH and β-GP concentration. The prepared thermoreversible hydrogels sustained vancomycin release for 21 days. Released vancomycin concentrations ranged from 3 to 95 folds the reported minimal inhibition concentration (MIC 90) for MRSA at all release study time points. Chitosan/β-glycerophosphate thermoreversible hydrogels were enzymatically degradable and 17.57% of the gel weight remained after 21 days. The preserved antimicrobial activity found after release of the antibiotic from the hydrogel elected this delivery system for further in-vivo and toxicological investigations.
Hend Mohamed Abdel-Bar, Amal Youssef Abdel-Reheem, Rihab Osman*, Gehanne A.S. Awad and Nahed Mortada
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
01 February, 2014
01 February, 2014
24 March, 2014
01 July, 2014