ORAL NANO-EMULSION OF FENUGREEK OIL FOR TREATMENT OF DIABETES

Diabetes mellitus (DM) is one of the principal causes of death in the world. DM causes many diseases with different pathogenesis disturbing glucose, protein and lipid metabolism. The primary characteristic is elevated glucose, blood level, which may be due to deficiency in the secretion of insulin and / act. Fenugreek oil is covered to recover glucose levels, intolerance, and insulin sensitivity as compared to the diabetic group. It is claimed that there is less damage of β-cell of pancreatic islet after dosing of fenugreek oil in diabetic rats. The objective of the study was to formulate a nano formulation of fenugreek oil, so as to enhance the effectiveness of fenugreek oil in treating induced diabetes by intraperitoneal (IP) injection of streptozocin (45 mg / kg) in Wistar albino rats, since nano formulation is claimed to enhance the bioavailability of bioactive compounds. A nano-emulsion was developed by adopting emulsion phase inversion and emulsion titration technique. The nano-emulsion with droplet size with less than 100 nm in diameter was stabilized by non –ionic surfactants. Ternary systems of three components made up fenugreek oil, Tween 80 and distilled water were set up at different weight ratios and mixed via vortex agitation. From this study it was clear that the developed nano formulation has considerably reduced the glucose level in blood in the diabetic rats, whereas there is no reduction in cholesterol, HDL cholesterol, blood level in diabetic rats, whereas a significant reduction in LDL cholesterol level is realized.