PAN-GENOMIC ANALYSIS OF HUMAN INFECTING SEROTYPES OF LISTERIA MONOCYTOGENES: IDENTIFICATION OF PUTATIVE DRUG TARGETSAbstract
Pan-genome analysis can identify the core genome, which is the total number of genes present in all strains of a bacterial species. Further essential genes can be identified in this core set of genes which gives us a minimal number of genes required for the survival of the bacteria. This set of genes can be analyzed to develop new antimicrobial agents against pathogenic, multidrug-resistant bacteria such as Listeria monocytogenes which causes several primary and secondary infections in humans. Pan-genome analysis of 38 strains of Listeria monocytogenes was performed to estimate 2331 genes in core genome, 1056 genes in the dispensable genome, and 152 genes in the strain-specific genome. Essentiality analysis of 2331 core genome proteins predicted 965 essential core-genome families (ECGFs). Furthermore, the identification of host non-homologous proteins using BLASTP with the Homo sapiens proteome resulted in 485 non-homologous essential core-genome families (NH-ECGFs). These putative proteins can be analyzed to identify novel drug targets that could generate broad-spectrum, safe and effective therapeutic agents against Listeria monocytogenes infections in humans.
Niharika Chandra, Yamini Chand, Tabish Qidwai and Sachidanand Singh *
Department of Biotechnology, Smt. S. S. Patel Nootan Science & Commerce College, Sankalchand Patel University, Visnagar, Mehsana, Gujarat, India.
31 January 2023
23 March 2023
26 April 2023
01 September 2023