PHARMACOGENETIC QUALITY ASSESSMENT WITH IN-VITRO ANTIOXIDANT, ANTI-INFLAMMATORY, CYTOTOXICITY, AND ANTI-BACTERIAL POTENTIAL AGAINST PERIODONTAL PATHOGENS

Background: Herbal extracts have beneficial effects on humankind, so scientific research on their characteristics is necessary. Objective: Our investigation aims to analyze the pharmacognostical, phytochemical constituents, standardization, free radical scavenging activity, in-vitro anti-inflammatory activity, and cytotoxicity activity of the HAE of Ashvakatri, an herbal drug. Material and Method: The studies were based on botanical and pharmacognostical investigation, including standardization, chromatographic evaluation, absorption maxima, and compatibility of the drug, in-vitro antioxidant evaluation, anti-inflammatory evaluation, and brine shrimp lethality assay were performed. Results: Microscopic studies of rhizomes revealed the presence of multicellular trichomes, ground tissue, epidermis, dictyosome, tracheids, and phloem. Likewise, the powder microscopy verified the presence of parenchyma cells, trichomes, tracheids, epidermal cells, etc. Proximate analysis was evaluated by total ash value, acid-insoluble ash value, and water-soluble ash value. The HAE yield was found to be 22.839% w/v. The phytochemical screening in HAE revealed the presence of alkaloids, tannins, glycosides, flavonoids, polyphenols, gums, and mucilage. The chromatographic evaluation of HAE was performed and resulted in the presence of alkaloids, flavonoids, glycosides, and tannins. Significant anti-oxidant and anti-inflammatory properties as compared to standard drugs were evaluated. The LC50 value of cytotoxicity was found to be more than threefold higher than the standard. Also, the MIC of HAE was assessed against periodontal pathogens and compared with the standard. Conclusion: Our investigation concluded that pharmacognostical, followed by anti-oxidant assay, anti-inflammatory activity, cytotoxicity, and antibacterial assay, provided a promising claim for potent activities of Ashvakatri against Periodontitis.