PHARMACOGNOSTIC EVALUATION OF SETARIA ITALICA L. CARYOPSIS – A NUTRITIONAL AND THERAPEUTIC MILLET IN AYURVEDA
HTML Full TextPHARMACOGNOSTIC EVALUATION OF SETARIA ITALICA L. CARYOPSIS - A NUTRITIONAL AND THERAPEUTIC MILLET IN AYURVEDA
Talat Anjum, Sonam Tamchos, Sanjay Kumar Singh and Subash Sharma
Regional Ayurveda Research Institute (CCRAS), Bantalab, Jammu, Jammu and Kashmir, India.
ABSTRACT: Setaria italica L. is one of the world’s oldest crops, which has been cultivated and domesticated about 8000 years ago. It is known for its ecological, nutritional and medicinal properties. In Ayurveda, it is called kangu/priyangu and is believed to nourish the body tissues and absorb excessive fluids besides, widely used in the treatment of various disorders. Since, no reports are available on microscopical and phytochemical studies of the caryopsis of Setaria italica L., hence, the present study was undertaken to investigate the same. Pharmacognostic and preliminary phytochemical study of the caryopsis of Setaria italica L. were carried out using microscopy, physicochemical analysis and TLC. The microscopy shows the presence of tube cells and cross cells, starch grains devoid of hilum, and occurring singly or in aggregates, tangentially elongated cells of pericarp, and endospermic cells filled with aleurone grains. The physicochemical analysis shows 8.33% w/w LOD, 3.10% w/w, Ash value and 7.83pH.TLC did not show any colour at UV 254 and 366 nm however, three brownish colour bands of Rf values (0.4, 0.44, and 0.47) are found in derivatised with Vanillin-Sulphuric acid. The result obtained from pharmacognostic studies and chemical fingerprints could be used as standardization data of the caryopsis of Setaria italica L., to apply or provide for guarantee of its quality.
Keywords: Setaria italica, Ayurveda, Pharmacognosy, TLC, Microscopy
INTRODUCTION: Foxtail millet (Setaria italic L.) is a member of Poaceae family. It is an annual, erect herb, 0.6-1.5 m tall, widely cultivated in many parts of temperate and tropical countries of Africa, America and Asia 1-2. The plant is native of China, and it is regarded as one of the world’s oldest cultivated crops that was domesticated about 8000 years ago 3. It is considered as one of the important millets which is drought resistant, capability to survive under stressful climatic conditions, resistance to pests and diseases, matures in shorter period than cereals 4-5.
The crop is adapted to different biotic and abiotic factors like salinity and fungal infection 6-7. Due to some distinct characteristics, such as short life cycle, short height, self-compatibility, adequate seed production per plant, true diploid nature (2n = 18), and small genome size make Setaria italica L. and its ancestor Setaria viridis (green foxtail) a perfect model plant to many scientists 3. Like maize and sugarcane, foxtail millet and green foxtail are typical C4 plants and are used for the study of photosynthesis by many researchers 1, 6, 8-11.
Foxtail Millet is nutritionally and medicinally important as it is rich in antioxidants and numerous macro- and micronutrients. Presence of these required nutrients makes it potential dietary supplement 12-13. Ayurveda classics classify millets among a specific group of Dhanya, i.e., Kshudradhanya or Kudhanya, or Trundhanya. This Dhanya Varga (group of grains) encompasses the properties of having Kshaya, Madhura Rasa, KatuVipaka, Sheeta Veerya, and Ruksha Guna. Owing to these Gunas, the Kledshoshak (absorption of excess fluid) and Pitta raktaka phahara properties are predominant, along with Vatkrit (aggravation of Vata) and Baddhvitaka (causing reduction in faeces) actions 14. Kangu Dhanya/Priyangu is one among these, which possesses the Bhagnsandhankara (fracture healing), Brumhana (nourishment of body tissues) 15-16, Durjara (difficult to digest) 16 and Sleshmahra (reduces Kapha) properties as a result of its Ruksha nature.
It has good nutritional, nutraceuticals and bioactive components 17. The Phytochemical compounds such as dietary fibre, fatty acid, resistant starch, phenolic compounds, phytosterols, tocopherol present in this millet are responsible for bioactive properties 18. The Foxtail Millet is high in Vitamin B12, which is important for maintaining a healthy heart and nervous system. It also aids in improving the state of glycemic control and the reduction of insulin, cholesterol, and fasting glucose in Type 2 diabetes 19. In ethnobotany, it is used in the treatment of measles and chicken pox 20. In present paper pharmacognostic study of caryopsis has been reported for the first time. Ultrastructure and powder characters of this valuable millet have been highlighted for its identification in future.
Morphology of Fruit: Fruit is a ridged caryopsis upto 3.0 mm long and 0.5 mm – 1.0 mm wide, narrow, ellipsoidal tapering at both ends. Surface is hispid hairy and consist of tightly enclosed boat shaped pericarp which is 5-nerved, golden yellow or yellowish brown in colour. Lemma is usually awned and edges of awns are rough or barbed. Seed is smooth, oval to oblong, oblique enclosing very small embryo on the ventral side.
MATERIALS AND METHODS
Collection and Authentication of the Sample: Authentic sample of dried seed of Setaria italica L. was procured from CARI Bangalore, one of the peripheral Institutes of Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Govt. of India. Botanical binomials and family details of the whole plant were confirmed from the Flora British India 21. Sample has been preserved in RARI, Jammu Museum bearing accession number RARI-JM-078.
Section Cutting: The dried caryopsis was soaked in water for 15-30 minute for softening the outer tissue. Transverse sections were hand cut with a sharp razor blade.
Preparation of Powder: Fine powder was prepared from the dried sample using a mixer grinder. The powder was strained using 60 No. sieve (as per the standard procedure). The final filtered powder was then used for the study.
Microscopy and Powder Analysis: Pharmacognostic studies were carried on dried raw drug samples by standard method as mentioned in Ayurvedic Pharmacopoeia of India, Part-I, Vol-I, Ministry of AYUSH, Govt. of India. The macroscopic features were noted as visualised with naked eye and stereo-microscopic observations using Olympus SZ2-ILST. Microscopy of the sample was carried out by standard methods under different magnifications using Olympus trinocular microscope CX41. Free hand sections were cleared with distilled water and observed under microscope. No stains were used, photographs of magnifications of the figure are represented by the scale-bars. The histochemical studies were carried out for the sample tissues and microphotographs were taken. Powder microscopy was carried out to observe the diagnostic character of sample.
Physicochemical Analysis: Physicochemical parameters like pH, Loss of drying, Ash, Aqueous soluble extractive value, Alcohol soluble extractive value, Acid insoluble ash and foreign matter were computed according to the method prescribed in Ayurvedic Pharmacopoeia of India (API).
TLC Methodology: 4 g of sample ware soaked overnight with 40 ml of ethanol, boiled, filtered and made up to 10 ml volumetric flask. The sample solution 5 µl, 10 µl was applied on Tracks-1& Track-2 respectively on an E. Merck aluminium plate pre-coated with Silica gel 60F254 of 0.2 mm thickness using ATS4 applicator. The plate was developed in the solvent system of Toluene: Ethyl acetate = 9:1 upto 90 mm and dried. The plate was observed through CAMAG TLC Visualizer under UV at 254 nm and 366 nm photos were taken.
Finally, the plate was dipped in Vanillin-Sulphuric acid reagent and heated in hot air oven at 105 oC untill the colour of the spots appeared, and photo was documented.
RESULTS AND DISCUSSION:
Macroscopy of the Caryopsis: The caryopsis of Setaria italica L. has following diagnostic features Fig. 1.
FIG. 1: CRYOPSIS OF SETARIA ITALICA L. AND ITS POWDER
Appearance: A ridged caryopsis narrow, ellipsoidal, surface hispid hairy; lemma usually awned, seed smooth.
Shape: Oval to oblong, oblique
Size -2.8-3.0mm long and 0.5mm – 1.0mm wide
Texture: Smooth
Microscopic (Seed): T.S. of Setaria italic L. caryopsis is more or less heart shaped in outline and shows outermost layer of pericarp, followed by aleurone layer encircling the endosperm.
Detailed T.S of the caryopsis of S. italica shows outer coat composed of epidermis of the pericarp covered with cuticle. It is followed by tube cells and cross cells. Cross cells are oriented transversely to the long axis of the caryopsis and tube cells lie parallel to the long axis. Both these cells have thick walls with simple pits. The testa or seed coat is reduced to a thin layer, brownish in colour and it is followed by aleurone layer composed of a single layer of cubical cells which envelops endosperm. Endosperm and embryo occupy most part of the grain. Endosperm consists of polygonal cells packed with starch grains. The starch grains are oval, small without any striations and lack a hilum Fig. 2. The surface view of the epidermis shows parallel rows of sclerified cells, composed of tube cells and cross cells Fig. 2.
Powder Analysis:
Organoleptic Study: Powder of S. italica L. is smooth in texture and appears dark creamish in colour. Odour is pleasant and tastes sweet.
Microscopy: Powder of S. italica L. shows single and compound starch grains oval in shape and with narrow lumen. Groups of tangentially elongated cells of pericarp, tube cells and cross cells in surface view and fragments of endospermic cells filled with aleurone grains Fig. 3.
FIG. 2: T. S (A-D) AND SURFACE VIEW (E-G) OF THE CARYOPSIS OF SETARIA ITALICA L. THE OHOTOGRAPH OF THE CARYOSIS INCLUDES PERICARP (PER), ALEURONE LAYER (SLEUR); CUTICLE (CU), EPIDERMIS (E), ENDOCARP (ENC), TUBECELL (TU), ENDOSPERM (END), CROSSCELL (CC), PERICARP (PER), SCUTELLUM (SCUT), RADICLE (RAD), ALEURONE GRAINS (AG) AND STARCH GRAIN (SG)
FIG. 3: MICROSCOPIC CHARCTERS OF SETARIA ITALICA L.
Physicochemical Properties: The results of physicochemical analysis of S. italica L. are mentioned in Table 1. The foreign matter is present < 2%; whereas the Loss on drying, ash value and Acid insoluble ash were calculated 8.33%, 3.10% and 1.47% respectively. The Extractive value for aqueous and alcohol were 3.76% and 5.45% respectively.
TABLE 1: PHYSICOCHEMICAL CONSTANTS OF S. ITALICA L.
S. no. | Test parameter | Results of Setaria italica L. in % w/w |
1 | Foreign matter | Less than 2 |
2 | LoD | 8.33 |
3 | Ash value | 3.10 |
4 | Aqueous soluble extractive value | 3.76 |
5 | Alcohol soluble extractive value | 5.45 |
6 | Acid insoluble ash | 1.47 |
7 | pH (4% aqueous solution) | 7.83 |
Thin- Layer Chromatography: Results of TLC reveals the Rf values and colour of the resolved bands Fig. 4A. No Rf value and colour was indicated in 254 and 366 nm of UV light. However, three Rf values (0.40, 0.44 and 0.47) and brownish colour were Derivatised with Vanillin-Sulphuric acid for the ethenol extract of seed of Setaria italic L. Both the tracks show similar band, and all three spots are found below 0.5 Fig. 4B.
FIG. 4: (A) TLC IMAGE OF SETARIA ITALICA L. CARYPOSIS EXTRACT AND (B) RF VALUE OF EXRTRACTS
CONCLUSION: Keeping in view the importance of millets due to its nutritional value and health benefits as mentioned in Ayurveda and growing use to beat lifestyle disorders, standardization of this important millet is essential for ensuring its purity, quality and sample identification. Macroscopy and microscopy along with the Quantitative analytical microscopy are one of the simplest and cheapest methods for establishing the correct identity of the source material.
ACKNOWLEDGEMENT: The Authors are thankful to Dr. Rama Rao, CARI Bangalore for supplying the raw material for this study.
Financial Support and Sponsorship: Director General CCRAS, Ministry of AYUSH, Government of India, New Delhi for funding project.
CONFLICTS OF INTEREST: There are no conflict of interest
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How to cite this article:
Anjum T, Tamchos S, Singh SK and Sharma S: Pharmacognostic evaluation of Setaria italica L. caryopsis - a nutritional and therapeutic millet in Ayurveda. Int J Pharm Sci & Res 2024; 15(8): 2360-64. doi: 10.13040/IJPSR.0975-8232.15(8).2360-64.
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IJPSR
Talat Anjum, Sonam Tamchos, Sanjay Kumar Singh and Subash Sharma
Regional Ayurveda Research Institute (CCRAS), Bantalab, Jammu, Jammu and Kashmir, India.
talatanjum11@gmail.com
05 March 2024
27 March 2024
19 April 2024
10.13040/IJPSR.0975-8232.15(8).2360-64
01 August 2024