PHARMACOLOGICAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED USING STEMODIA VISCOSA AQUEOUS EXTRACT

The Pharmacological effects of silver nanoparaticle of Stemodia viscosa aqueous plant extract on albino wistar rats was explored. The plant extract 40 ml was added to 10 ml of 10mM silver nitrate aqueous solution and kept at room temperature for synthesizing silver nanoparaticle. Diabetes was induced in overnight fasted mice by single intra-peritoneal injection of 55 mg/kg of Streptozotozin (STZ), freshly dissolved in 0.1M cold citrate buffer, pH 4.5. After five days of STZ administration, blood was collected and plasma glucose levels were determined. For wound healing activity, a control ointment base was formulated without any drug content. Two creams were formulated by using 5% extract were incorporated in 100gm of cream base. The observation of the percentage wound contraction were made on 4^th , 8^th , 12^th , 16^th and 22^nd day of  post wounding days. All the values were statistically   analyzed by unpaired student test comparing with control. The administrations of plant extract to STZ induce diabetic mice, significantly lowered the plasma glucose level as compared to diabetic control. The study is further, extended to analyze lipid profile which was significant that Streptozotocin induced mice was found to be higher with 67.05 mg/dl than the normal control mice. The ointment formation were prepared from the plant extract the results given in that wound contracting ability of Stemodia  viscosa  ointment (5% w/w) was found significant than the control. Stemodia viscosa exhibit a remarkable wound healing and anti-lipedmic activity.