PHARMACOLOGICAL ASSESSMENT OF ANTI-ANAEMIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF CURCUBITA PEPO SEEDS AND MACROTYLOMA UNIFLORUM GRAINS IN RATS

This study aimed to assess the anti- anemic activity of Macrotyloma uniflorum grains and Curcubita pepo seeds against Phenylhydrazine- induced anemia in albino rats. It was done by two extraction methods. In extract-1, Macrotyloma uniflorum was collected and soaked in water for whole night and next morning the grains were tied in a clean cloth and left for 3-4 days (Sprouted). The sprouted grains were dried under shade. Then the sprouted grains are fine powdered in a mill. The powder was macerated with 50% of alcohol for 7 days and filtered. The filtrate was evaporated after the complete evaporation of the crude extract. This extract was given to the animals for 14 days and on 15^th day, the blood samples were collected and examined. In extract-2, Curcubita pepo was collected, outer shell was removed and dried under shade. The dried material was fine powdered in a mill. The powder was macerated with 50% of alcohol for 7 days and filtered. The filtrate was evaporated. After the complete evaporation of the crude extract. The extract was given to the animals for 14 days and on 15^th day the blood samples are collected & examined. Both groups’ blood samples are examined for Hb, PCV, MCH, MCHC, RDW, RBC, WBC were considered to determine the anti-anemic activity. Hydroalcoholic extract of Macrotyloma uniflorum and Curcubita pepo has increased the anemic condition of rats to normal level and showed anti-anemic activity against Phenyl hydrazine- induced anemia by significant increase in the levels of RBC.