PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY STUDIES OF LEAF OF BRASSICA JUNCEA VAR RUGOSA THROUGH SEQUENTIAL SOLVENT EXTRACTION

Objectives: To find out different qualitative and quantitative phytochemical properties, it’s in-vitro antioxidant properties and peroxidase activity of Brassica juncea var. Rugosa (record tro- 50197928) with fresh and sequential solvent extracts. Methods: The leaf sample of Brassica juncea var. rugosa was collected from Manipur and the sample was dried. The dried powder was extracted using Soxhlet with the sequential solvent of non-polar to polar. Preliminary phytochemical analysis for proteins, carbohydrates, phenols, alkaloids, flavonoids, tannins, saponins, steroids, terpenoids, and quantitative estimation for total proteins, carbohydrates, alkaloids, phenols, flavonoids, and tannins. In-vitro, antioxidant activity was analyzed by using DPPH assay. Peroxidase activity was also checked. Results: The plant extracts showed the presence of a considerable amount of total alkaloids, total phenols, total flavonoids, total tannins, total proteins, total carbohydrates. The plant extracts also showed high antioxidant and peroxidase activity. Conclusions: From the study, we can conclude that the plant Brassica juncea var. rugosa has many important secondary metabolites and it has a potential for strong antioxidants.