PHYTOCHEMICAL AND ANTIOXIDANT ASSAY OF ECLIPTA ALBA (L.) LEAF EXTRACT

Currently, pharmacological activities of Eclipta alba (L.) plant extracts and individual phytoconstituents have revealed anticancer, hepatoprotective, snake venom neutralizing, anti-inflammatory and antimicrobial properties. The role of antioxidants is increasing day by day due to their multiple roles to reduce the harmful effects of oxidative stress. Phytoconstituents like wedelolactone and ursolic and oleanolic acids as well as luteolin and apigenin can form the basis of new drugs against cancer, arthritis, gastrointestinal disorders, skin diseases, and liver disorders. Plants have all these activities due to biologically active compounds, and for this, we have analyzed phytochemical screenings and antioxidant activity through the DPPH, ABTS●+, and reducing power assay and we got maximally phenols then flavonoids and flavonols and a good natural antioxidant agent. The best-known compound in E. alba i.e., Wedelolactone. It was analyzed by TLC, and it was present as 0.52 R_f value and present in pet ether extract and acetone extract and minimum in ethanol extract. The antioxidant activity was assessed through DPPH, ABTS●+ free radical scavenging activity and reducing power assay, this was explained in terms of effective concentration EC₅₀ / IC₅₀ and Anti-oxidant Radical Power (ARP) values. The maximum free radical scavenging activity was showed in pet ether compared to other extracts.