PHYTOCHEMICAL AND TOXICOLOGICAL INVESTIGATION ON AQUEOUS EXTRACT OF POLYHERBAL PREPARATIONAbstract
Polyherbal preparations (PHP) are known to express high effectiveness in a vast number of diseases. In view of this, present investigation was carried out to identify phytochemical constituents of PHP, prepared from the aqueous extract of test plant powders of Mimusops elengi Linn., Strobilanthes barbatus Nees, Indigofera zollingeriana Miquel and Dillenia indica Linn. Phytochemical screening of APHP revealed the presence of carbohydrates, Proteins and amino acids, alkaloids, glycosides, phenols, flavonoids, and fixed oils/fats. Acute toxicity studies were conducted on female wister rats. Study design is according to OECD Guidelines no. 423. APHP was administered at dose of 5 mg/kg, 50 mg/kg, 250 mg/kg, 500 mg/kg, 1000 mg/kg and 2000 mg/kg body weight as suspension (1% Na CMC) along with blank. No-Observed-Adverse-Effect- Level (NOAEL) of APHP was found to be 2000 mg/kg. In connection to that, sub chronic toxicity studies have been carried out to predict the dose to be selected for in-vitro and in-vivo experiments. APHP at the doses of 250 mg/kg, 500mg/kg and 1000mg/kg were administered and examined for variations in serum biochemical parameters, haematological parameters, body weight and organ weight. Data obtained in this study indicated no significant signs of any toxicity due to administration of APHP at the doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg to rats.
R. Pasupula * and R. R. Pragada
Department of Pharmacology, St. Anns College of Pharmacy, Prtakasam, Vizianagaram, Andhra Pradesh, India.
26 August, 2017
20 November, 2017
25 December, 2017
01 July, 2018