PHYTOCHEMICAL INVESTIGATION, ANALGESIC AND ANTIPYRETIC ACTIVITIES OF ETHANOLIC EXTRACT OF KARIYAT

An ethanolic extract of Kariyat (Andrographis paniculata) was prepared by using Soxhlet extractor. Kariyat ethanolic extract (KEE) was subjected for phytochemical investigations and screened for analgesic and antipyretic activities in experimental animals. High performance thin layer chromatography (HPTLC) and gas chromatography mass spectrometry (GC-MS) investigations indicated presence of steroids, phenols, terpenoids, alkaloids, saponins, flavonoids etc. The various concentrations ethanolic extract were tested for analgesic activity in mice by using acetic acid-induced writhing in mice and hot-plate method. The maximum protection of 56.23 % against writhing movement was observed at 300mg/kg when compared to standard drug which showed 79.43% protection. Analgesic activity of KEE using hot plate method indicates mean basal reaction time increased significantly in the treated group compared to control group from 30 min onwards. The various concentrations of KEE also tested for antipyretic activity in rats by using Brewer’s Yeast Induced Hyperpyrexia and results significant antipyretic activity 1 hr after drug administration. Mechanism of action was determined by studying prostaglandin inhibition studies. Results indicated that KEE produces action by inhibiting the synthesis of prostaglandin.  The findings of present study indicate ethanolic extract of Kariyat shows significant analgesic and antipyretic activity.