PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITIES OF SOME WILD MEDICINAL PLANTS OF THE WESTERN MEDITERRANEAN COASTAL REGION, EGYPTAbstract
Four wild plant species (Chrysthanthemum cornarium, Capparis spinosa, Carthamus lanatus, Haplophyllum tuberculatum) were investigated for their antioxidant activity, phenolic and flavonoid contents of their methanolic extracts. The antioxidant activity of the extracts was assessed their free radical scavenging properties using DPPH. Total phenolics and total flavonoids were detected by spectrophotometric and HPLC methods. The HPLC analysis compared with 14 reference standard phenolic compounds, results confirmed that gallic acid, chlorogenic acid, caffeic acid, 2,5- dihydroxy benzoic acid, and catechin were detected in the four methanolic plant extracts. HPLC chromatograms of the plant extracts showed that there was noticeable valuation in the distribution of phenolic compounds in the four studied species. Six bacterial strains were used in this study, three Gram-positive and three Gram-negative. Concerning with antimicrobial activity, results showed that plant extract of the studied species are a great source of phenolic compounds and represents the highest antibacterial activity against Gram-positive and negative bacteria. Results revealed that extracts represent the highest antibacterial activity against Gram-positive and negative bacteria and largest inhibition zone for bacterial strains were exhibited by the extracts of Haplophyllum tuberculatum (79 mm) followed by that of Capparis spinosa (54mm). However, Chrysthanthemum cornarium extract showed the least inhibition zone (34 mm).
H. M. Abou-Zeid *, L. M. Bidak and Y. M. Gohar
Department of Botany & Microbiology, Faculty of Science, Alexandria University, Egypt.
02 April 2014
21 May 2014
17 July 2014
01 October 2014