PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF HEXANOLIC EXTRACT OF LANTANA CAMARA LINN.HTML Full Text
PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF HEXANOLIC EXTRACT OF LANTANA CAMARA LINN.
Mamta Uparkar * and Sunil H. Ganatra
Department of Chemistry, Institute of Science, Civil Lines, Nagpur - 440001, Maharashtra, India.
ABSTRACT: Lantana camara Linn. is an important ethnomedicinal plant with several medicinal properties. It is widely used in the traditional medicinal system as carminative, anti-inflammatory, antipyretic and respiratory system infections. The present investigation was carried out to determine the qualitative analysis of phytochemical screening and the presence of bioactive compounds from the hexanolic extract of Lantana camara leaves using GC-MS. The GC-MS analysis of hexanolic extract reported the presence of cis-Caryophyllene, caryophyllene oxide, isophytol acetate, 13-docosenamide, (Z) - and Squalene as major compounds. The presence of terpenes in extract confirms its application as anti-cancer, antioxidant and anti-microbial agents along with traditional medicine application.
Lantana camara Linn., Terpenes, Anti-cancer agent, GC-MS, Phytochemical
INTRODUCTION: Medicinal plants are a treasure house of many potential drugs. There has been an increase in awareness and interest in the importance of medicinal plants in recent years. Drugs obtained from the medicinal plants are easily available, cheaper, safe and having minimum side effects. According to WHO, 80% of the world’s population from developed countries depend on traditional medicines for primary health care 1. Medicinal plants contain bioactive compounds that participate in physiological action on the human body 2. Lantana camara Linn. is listed as one of the most important medicinal plants of the world 3. It is a significant weed with 650 varieties growing in 60 countries.
It is commonly known as wild or red sage and regarded both as a notorious weed and ornamental garden plant and it belongs to family Verbenaceae 4. In India, it was introduced at the National Botanical Gardens, Calcutta in 1807 as an ornamental plant 5. Lantana camara Linn. is an evergreen strong aromatic, hairy shrub. It grows to a height 1 to 3 meters and it can spread to 2.5 meters in width. The stems and branches are sometimes armed with prickles or spines. The leaves are arranged in opposite pairs, elliptic about 3 inches long and 1.5 inches wide with pointed tip and base rounded and toothed in the margins.
Flowers are small held in clusters (called umbels) with various colors pink, red, orange, yellow, white or violet. The fruits are fleshy berries in clusters, shiny and globose in shape, green-colored, which changes to black on ripening 7, 8, 9. Lantana camara has been historically useful as a medicinal and ornamental plant for a very long time. All parts of this plant have been widely used in traditional medicines for the treatment of malaria, ulcers, cancer, high blood pressure, tetanus, tumors, eczema, cut, catarrhal infections, chickenpox, measles, rheumatisms, asthma, colds, fevers, swellings, etc. 10 Lantana camara has been scientifically proven to possess anti-bacterial, anti-fungal, anti-filarial, anti-hypertensive, hemolitic, anti-ulcerogenic, anti-inflammatory, anti-proli-ferative, anti-fertility and anti-oxidant activities 11. The plant has also been reported to possess antipyretic, anti-cancer, anti-mutagenic, anti-hyperglycemic, hepatic protective, larvicidal, termiticidal activities 12. The plant has been reported to contain the important phytochemicals such as essential oils, phenolic compounds, flavonoids, carbohydrates, proteins, alkaloids, glycosides, iridoid glycosides, phenylethanoid, oligosaccharides, anthraquinone, saponins, steroids, triterpenes, sesquiterpenes, tannins, etc. 13 it is reported that this plant contains the number of terpenoids. Nowadays, the number of anti-cancer drugs belongs to terpenoid class, like glycyrrhizic acid, Oleanolic acid, ursolic acid, Nomilin, etc. 14, 15, 16, 17, 18
|Species||:||Lantana camara Linn. 6|
FIG. 1: LANTANA CAMARA PLANT
MATERIAL AND METHOD:
Collection of Plant Material and Powder Preparation: The fresh leaves of Lantana camara were collected from Bela Village, near Nagpur city. This city is located at the central part of India.
The sample of plant was identified by senior plant taxonomist, Dr. S. U. Borkar, Department of Botany, Govt. Institute of Science, Civil Lines, Nagpur (Maharashtra), India. The specimen was submitted to the herbarium of the department with authentication code, IoSc./Bot.Sci/09/1/2018-19/192. The collected plant is shown in Fig. 1. The plant leaves were washed thoroughly with clean water to remove dust and dirt and then air-dried under shade at room temperature for about 15 days. The dried leaves were ground to a fine powder using pestle and mortar and a domestic electric grinder. The material was stored in an airtight brown color glass bottle for further use.
Preparation of Extract: The coarsely powdered plant material of Lantana camara was extracted with n-hexane (≥99% pure, Merck) solvent by cold (maceration) method. In each batch, 25 grams of plant material were dissolved in 250 ml of n-hexane solvent in airtight stopper brown bottle. After every eight hours, the bottle was shaken for 1 hour using a shaking machine. The process was repeated for 3 days. The solution was then filtered through Whatman filter paper no. 1. The filtrate was evaporated using a rotary evaporator and further dried at room temperature. The obtained dried crude extract was collected in an airtight container and stored in a refrigerator at 4 °C for further analysis.
Preliminary Phytochemical Analysis: The crude extract was subjected to the qualitative chemical tests for the detection of various phytochemicals using standard procedures as described by Harborne, Trease, Evans and Sofowora 19, 20, 21. The observations for chemical tests are shown in Table 1. It is reported that the extract contains flavonoids, saponins, terpenoids, carbohydrates and phyto-sterols in major quantities.
Gas Chromatography-Mass Spectrometry (GC-MS) Analysis: The GC-MS analysis was carried out using Thermo scientific TSQ 8000 GC-MS system and Gas Chromatograph interfaced to a mass spectrometer (GC-MS) instrument employing the column TG5MS (30 m × 0.25 mm, 0.25 µm, composed of 100% dimethylpolysiloxane). For GC -MS detection, an electron ionization system with energy of 70 ev was used. Helium gas (99.99%) was used as the carrier gas at a constant flow rate of 1ml/min. The oven temperature was raised from 60 °C to 280 °C (raising at the rate of 10 °C/min). The total GC running time was about 31 min. The mass spectra were carried out coupled with Gas chromatography by maintaining 70 ev voltage and keeping the source temp of 230 °C.
The inlet line temperature was maintained at 280 °C and the mass scan rate was tuned to 50-700. The total MS running time was about 34 min.
TABLE 1: PHYTOCHEMICAL SCREENING OF HEXANE EXTRACTS OF LANTANA CAMARA LEAVES
|S. no.||Phytoconstituents||Test Procedure 19, 20, 21||Observation in Hexanoic Extract|
|1||Alkaloids||2 ml of the extract was treated with a few drops of Hager's reagent (picric acid dissolved in benzene). The yellow precipitate was not formed, indicating the absence of Alkaloids||-- (Negative Result)|
|2||Flavonoids||1 ml of the extract was treated with 10% of 1 ml Pb(OAC)4 . Formation of intense yellow colour indicates the presence of Flavonoids||++ (Positive Result)|
|3||Phenols||2 ml of the extract was treated with 3 to 4 drops of FeCl3 solution. The extract was not converted to bluish-black colour, indicating the absence of phenols.||-- (Negative Result)|
|4||Saponins||To 5 ml of extract, a drop of sodium bicarbonate solution was added. The test tube was shaken and allowed to stand for 3 min. Formation of honeycomb-like froth indicates the presence of Saponins.||++ (Positive Result)|
|5||Terpenoids||2 ml extract was treated with 2 ml (CH3CO)2O and 2 to 3 drops of conc H2SO4. The red colour appeared, indicating the presence of Terpenoids||++ (Positive Result)|
|6||Carbohydrates||1 ml of the extract was mixed with 2 drops of alcoholic (ethanolic) naphthol solution in a test tube and was shaken properly. After that, 2 ml of conc. H2SO4 was added carefully along the side of the tube. Violet ring formed at the junction indicating the presence of Carbohydrates||++ (Positive Result)|
|7||Tannin||1 ml of the extract was treated with 2 to 3 drops of FeCl3 solution. The green precipitate was not observed, indicating the absence of Tannin.||-- (Negative Result)|
|8||Coumarins||2 ml the extract was treated with 3 ml of 10% NaOH solution. The solution didn’t turn yellow indicating the absence of Coumarins.||-- (Negative Result)|
|9||Glycosides||Liebermann's test: 2 ml the extract was mixed with 2 ml of chloroform and 2 ml of acetic acid. There is no change in colors, indicating the absence of Glycosides.||-- (Negative Result)|
|10||Anthraquinones||Borntrangers test: 3 ml of the extract was treated with 3 ml of benzene and 5 ml of 10% NH3. No change in color in ammonical layer indicating the absence of Anthraquinones.||-- (Negative Result)|
|11||Phytosterols (Salkowski’s test)||Salkowski test: Extract was treated with 2 ml chloroform and then filtered The filtrate was treated with few drops of H2SO4, the solution was shaken gently and allowed to stand. Golden color (reddish-brown) appeared, indicating the absence of Phytosterols||++ (Positive Test)|
RESULTS AND DISCUSSION:
Phytochemicals Extraction and Analysis: The preliminary phytochemical screening of the hexanoic extract of Lantana camara leaves revealed the presence of flavonoids, carbohydrates, steroids, saponins and terpenoids. Among the variety of secondary metabolites in Lantana camara, terpenoids are predominant compounds present in it. The presence of the high amount of terpenoid compounds revealed that Lantana camara may have anti-cancer activity. Table 1 shows the results of the phytochemical screening of hexanolic extract of Lantana camara leaves.
Gas Chromatography-Mass Spectrometry (GC-MS): The obtained spectrum of GC-MS analysis of hexane extract of leaves of Lantana camara is shown in Fig. 2. The spectrum shows more than 19 prominent peaks having higher retention time. Interpretation of the mass spectrum of GC -MS was conducted using the database of the National Institute of Standard and Technology (NIST). The spectrum of the unknown components was compared with the spectrum of the known components stored in the NIST library. Retention time, molecular weight, molecular formula and percentage composition were used to identify the components. A list of identified compounds and their retention time along with molecular formula are listed in Table 2.
FIG. 2: GC-MS CHROMATOGRAM OF HEXANE EXTRACT OF LANTANA CAMARA LEAVES
TABLE 2: COMPOUNDS IDENTIFIED IN THE HEXANOIC EXTRACT OF LANTANA CAMARA LEAVES BY GC-MS ANALYSIS
|S. no.||Name of compound||Retention time||Molecular formula||Molecular weight||Nature of compound|
|4||Bicyclo[7.2.0]undec-4-ene,4,11,11-trimethyl-8 methylene- ( cis-Caryophyllene)||10.14||C15H24||204.35||Terpenoid|
|8||1H-Cycloprop[e] azulen -7-ol,decahydro- 1,1,7- trimethyl-4–methylene-,[1ar-(1aa,4aa,7a,7aa,7ba)]-||11.49||C15H24O||220.354||Tricyclic sesquiterpenoid|
|9||Tricyclo[22.214.171.124(1,6)undecan-3-ol,2- methylene-6,8,8-trimethyl-||11.49||C15H24O||220.356||Tricyclic sesquiterpenoid|
|10||Caryophyllene oxide (beta-Caryophyllene epoxide)||11.55||C15H24O||22.0356||Bicyclic sesquiterpenes|
|11||Isoaromadendrene epoxide||11.55||C15H24O||220.356||aromadendrene sesquiterpenoid|
|14||3,7,11,15,-Tetramethyl- 2 -hexadecen -1- ol||15.11||C20H40O||296.53||Acyclic diterpene|
|15||Isophytol, acetate||15.11||C20H42O2||338.56||Terpenoid alcohol|
|17||trans -13- Docosenamide||19.67||C22H43NO||337.592||Amide|
|18||Bis(cis -13- Docosenamide)methane||19.67||C45H86N2O2||687.195||Amide|
The results pertaining to GC-MS analysis led to the identification of a mixture of numerous compounds. The majority among them belong to terpenoids. Table 3 shows the identified phytochemicals using GC-MS and their known biological activities.
TABLE 3: IDENTIFIED PHYTOCHEMICALS AND THEIR KNOWN BIOLOGICAL ACTIVITIES
|S. no.||Compound||Biological Activity 22, 23, 24, 25|
|3||(-)-Spathulneol||Anti-fungal, Antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities|
|4||Caryophyllene oxide (β-Caryophyllene epoxide)||Inhibited potassium ion fluxes, Analgesic, Anti-inflammatory activity, and Anti-fungal activity|
|5||13-Docosenamide,(z)-||Antimicrobial, Antinociceptive and anti-inflammatory activities|
|6||Squalene||Anticancer, Antioxidant, Drug carrier, Detoxifier, Skin hydrating agent|
CONCLUSION: GC-MS analysis revealed the presence of many important terpenoids compounds in the hexanoic extract of leaf of Lantana camara. The major components of the extract are cis-caryophyllene, β-Caryophyllene epoxide, L-camphor, 13-docosenamide, (z) - and squalene.
In this investigation, cis-caryophyllene is uniquely identified as it is not reported by any literature. Also, the major compound belongs to terpenoids and hence it justifies the medicinal importance of Lantana camara leaves for the treatment of several ailments by traditional practitioners. Further, isolation of active phytoconstituents and subjecting it to biological activity from the plant will definitely give fruitful results.
It could be concluded that Lantana camara leaves is a rich source of important secondary metabolites like terpenes. It is a plant with phytopharmaceutical importance.
ACKNOWLEDGEMENT: The authors are thankful to Sophisticated Analytical Instrument Facility, CIL Punjab University, Chandigarh for CG-MS analysis.
CONFLICTS OF INTEREST: Nil
- Burton A, Falkenberg T, Smith M, Zhang Q and Zhang X: WHO Traditional Medicine Strategy 2014-2023. World Health Organization WHO Press Geneva 2013; 27-28.
- Aguwa CN and Nwankwo SO: Preliminary studies on the root extract of Naulea latifolia for antiulcer properties. Nig. J Pharmaceutical Sci 1988; 4(1): 16-23.
- Sharma OP, Singh A and Sharma S: Levels of lantadenes, bioactive pentacyclic triterpenoids in young and mature leaves of Lantana camara Aculeata. Fitoteapia 2004; 71: 87-491.
- Sharma O, Dawra R and Makkar H: Isolation and partial purification of lantana Lantana camara toxins. Toxicology Letters 1987; 37: 165-72.
- Thakur ML, Ahmad M, and Thakur RK: Lantana weed (Lantana camara Aculeata) and its possible management through natural insect pests in India. Indian Forester 1992; 118: 466-88.
- Mishra A: Allelopathic properties of Lantana camara, Intern Res J of Basic and Clinical Studi 2015; 3(1): 13-28.
- Oyedeji OA, Ekundayo O and Konig WA: Volatile leaf oil constituents of Lantana camara from Nigeria, Flavour Fragrance J 2003; 18: 384-86.
- Sundufu AJ and Shoushan H: Chemical composition of the essential oils of Lantana camara occurring in south China, Flavour and Fragrance Journal 2004; 19: 229-32.
- Basu S, Ghosh A and Hazra B: Evaluation of the antibacterial activity of Ventilago madraspatana Gaertn, Rubia cordifolia and Lantana camara Linn.: Isolation of emodin and physcion as active antibacterial agents, Phtyother Res 2005; 19: 888.
- Day MD, Wiley CJ, Playford J and Zalucki MP: Lantana current management, status and future prospects. Australian center for Inter Agricu Res Canberra 2003; 128.
- Barreto FS, Sousa EO, Campos AR, Costa IJ and Rodriguez GM: Antibacterial activity of Lantana camara Linn and Lantana montevidences brig extracts from carri-ceara, Journal of Young Pharmacists 2010; 2(1): 42-44.
- Kalita S, Kumar G, Logonathan K, Venkata K and Rao B: Phytochemical composition and In-vitro hemolytic activity of Lantana camara (Verbenaceae) leaves, Pharmacology Online 2011; 1: 59-67.
- Ghisalberti EL: Review Lantana camara (Verbenaceae) Fitoterapia 2000; 71: 467-86.
- Raphael TJ and Kuttan G: Effect of naturally occurring triterpenoids glycyrrhizic acid, ursolic acid, oleanolic acid and nomilin on the immune system. Phytomedicine 2003; 10: 483-89.
- Raphael TJ and Kuttan G: Effect of naturally occurring triterpenoids ursolic acid and glycyrrhizic acid on the cell mediated immune responses of metastatic tumor-bearing animals. Immunopharmacol Immunotox 2008; 30: 243-55.
- Singh GB, Singh S, Bani S, Gupta BD and Banerjee SK: Anti-inflammatory activity of oleanolic acid in rats and mice. J Pharm Pharmacol 1992; 44: 456-58.
- Kapil A and Sharma S: Anti-complement activity of oleanolic acid: an inhibitor of C3-convertase of the classical complement pathway. J Ph Pha 1994; 46: 922-23.
- Kapil A and Sharma S: Effect of oleanolic acid on complement in adjuvant- and carrageenan-induced inflammation in rats. J Pharm Pharma 1995; 47: 585-87.
- Harbone JB; Phytochemical methods, Chapman and Hall Ltd, London 1973; 49-188.
- Trease GE and Evans MD: A textbook of Pharmacognosy, 13th Baillier Tindal and Caussel London 1989; 144-48.
- Sofowora A: Medicinal plants and Traditional medicines in Africa, Spect Books Ltd, Ibadan, Nigeria 1993; 289-95.
- Medeiros: Chemical constituents and evaluation of the cytotoxic and antifungal activity of Lantana camara essential oils. Rev Bras Farmacog 2012; 22: 1259-67.
- Singh T, Singh RK and Malik P: Analgesic and anti-inflammatory activities of Annona squamosa Linn bark. J of Scientific and Innovative Research 2014; 3(1): 60-64.
- Chavan M, Wakde P and Shinde D: Analgesic and anti-inflammatory activity of caryophyllene oxide from Annona Squamosa, Phytomedicine 2010; 17(2): 149-51.
- Shareef: Antibacterial effect of ginger (Zingiber officinale) roscoe and bioactive chemical analysis using gas chromatography-mass spectrum. Orient J Chem 2016; 32(2): 817-37.
How to cite this article:
Uparkar M and Ganatra HS: Phytochemical screening and GC-MS analysis of hexanolic extract of Lantana camara Linn. Int J Pharm Sci & Res 2020; 11(5): 2112-17. doi: 10.13040/IJPSR.0975-8232.11(5).2112-17.
All © 2013 are reserved by the International Journal of Pharmaceutical Sciences and Research. This Journal licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License.
M. Uparkar * and S. H. Ganatra
Department of Chemistry, Institute of Science, Civil Lines, Nagpur, Maharashtra, India.
26 June 2019
29 October 2019
08 February 2020
01 May 2020