PHYTOCHEMICAL STUDIES, FTIR AND GC-MS ANALYSIS OF HARDWICKIA BINATA ROXB. (FABACEAE / CAESALPINIACEAE)

Hardwickia binata belongs to family Fabaceae / Caesalpiniaceae and commonly known as ‘Anjan’. The present study includes phytochemical screening, FTIR and GC-MS analysis. Shade dried powdered leaves, seed and husk of H. binata extracted in petroleum ether, chloroform, ethyl acetate, acetone, methanol, ethanol, distilled water using Soxhlet apparatus and used for phytochemical analysis. Crude powder of plant used for FTIR analysis and ethanolic extracts was analyzed using GC-MS. The phytochemical screening of H. binata revealed the presence of carbohydrates, proteins, amino acids, steroids, glycosides, flavonoids, lipids, tannins, phenolic compounds, quinones, mucilage, volatile oils, fats, and fixed oils whereas alkaloids, emodins and resins were not detected in any extracts of leaves, seed, and husk. The majorities of compounds are present in acetone, methanol, ethanol and distilled water and glycosides, lipids and mucilage are present in all extracts. FTIR analysis showed the presence of alcohol, phenols, amines, amides, carboxylic acids, aromatics, alkenes, alkanes, aliphatic amines, esters, ethers, alkynes, alkyl halides in leaves, seed and husk; saturated aliphatic found in only seed and aldehydes only in husk and GC-MS analysis confirmed ten different chemical compounds 1,1-diethoxy-ethane, tetradecamethyl-cycloheptasiloxane, linoleic acid, ethyl ester, ethyl oleate in leaves, seed and husk; hexadecanoic acid and ethyl ester found in only leaves and seed; 1-methyl-4 -(1-methylethyl)-Benzene, 2-methyl-5-(1-methylethyl)-Phenol, 3, 7, 11, 15-tetramethyl-2-hexadecen-1-o1 are found in only leaves; dodecamethyl-cyclohexasiloxane only in seed; thymol only in husk shows various biological activities. The different extracts of H. binata revealed several phytochemical components as a rich source of pharmaceutically and biologically important bioactive compounds that may build up new medicines with more efficiency.