PHYTOCHEMICALS, IN-VITRO CYTOTOXICITY, ANTIOXIDANT, AND ANTIMICROBIAL EVALUATION OF BARLERIA CRISTATA L: PHILIPPINE VIOLET

The current study aims to find out how effective different extractions of Barleria cristata leaves are at fighting cancer, free radicals, and bacteria. Physical and chemical qualities and preliminary phytochemical analysis will also be assessed. It also quantifies several active ingredients by total phenolic and flavonoid. Five leaf extracts of Barleria cristata were made with ethyl acetate, chloroform, acetone, ethanol, and water. They were then tested for their antioxidant potential using reducing power and free radical scavenging methods, such as DPPH and NO radicals. They were also tested for antibacterial activity against Gram-positive and Gram-negative bacteria. The MTT assay measured how successfully the medication killed MCF-7 breast cancer cell line and Hep-G2 hepatoma cancer cell line. We calculated IC₅₀. Initial phytochemical analysis was performed on all extracts. The total extract was abundant in protein, carbs, tannins, flavonoids, and phenolics. Acetone extract had higher phenolic and flavonoid components than ethyl acetate, chloroform, ethanol, and water. Barleria cristata acetone extract was antioxidant rich. Plant extracts also had antibacterial properties. Extracts inhibited MCF-7 breast cancer cell line and Hep-G2 hepatoma cancer cell line in-vitro. This study showed that Barleria cristata leaves can prevent cancer cell proliferation. It also revealed that leaf phytochemicals combat free radicals and bacteria well. These compounds could be used to produce novel antimicrobials or cancer medicines.