PHYTOCHEMISTRY AND PHARMACOLOGICAL ACTIVITIES OF PSIDIUM GUAJAVA: A REVIEW
HTML Full TextPHYTOCHEMISTRY AND PHARMACOLOGICAL ACTIVITIES OF PSIDIUM GUAJAVA: A REVIEW
Payal Mittal*, Vikas Gupta , Gurpreet Kaur , Ashish K Garg and Amarjeet Singh
Akal College of Pharmacy and Technical Education , Mastuana Sahib, Sangrur, India
UCER, Baba Farid University of Health Sciences , Faridkot, India
ABSTRACT
Guava (Psidium guajava Linn.) Family Myrtaceae is important plant used traditionally for medicinal purposes. Guava is rich in antioxidants compounds and contains a high level of ascorbic acid. The pharmacological actions and the medicinal uses of aqueous extracts of guava leaves in folk medicine include the treatment of various type of gastrointestinal disturbances such as vomiting, diarrhea, inhibition of the peristaltic reflex, gastroenteritis, spasmolytic activity, dysentery, abdominal distention, flatulence and gastric pain. The important active constituents are essential oils, flavonoids, carotenoids, polyphenolic compounds, pentacyclic triterpenoids, esters, and aldehydes etc. This paper explains the Evidence-based information regarding the phytochemistry and pharmacological activity of this plant.
Keywords:
Guava, Psidium guajava, Phytochemistry, Pharmacology |
INTRODUCTION: Guava (Psidium guajava Linn.), belonging to the Family Myrtaceae, is originated in the tropical South America 1and grows wild in Bangladesh, India, Thailand, Brazil, Florida, West Indies, California and also in several other countries 2 . Guava is rich in antioxidants compounds and contains a high level of ascorbic acid ranging from 174.2 to 396.7 mg/100 g fresh fruit 3. Myricetin and apigenin were reported to be 549.5 and 579.0 mg/kg dry weight, respectively 4. It plays a vital role in fulfilling the vitamin C deficiency among the people of the country since 100 g of fruit contains about 260 mg of vitam5, which is 2–5 times higher than the fresh orange. The wood is hard and tough 6, used as posts for rural house buildings.
The pharmacological actions and the medicinal uses of aqueous extracts of guava leaves in folk medicine include the treatment of various types of gastrointestinal disturbances such as vomiting, diarrhea, inhibition of the peristaltic reflex, gastroenteritis, spasmolytic activity, dysentery, abdominal distention, flatulence and gastric pain 7. These extracts have also been indicated to cause disturbances of the central nervous system: insomnia, convulsions and epilepsy 8. Bronchitis, asthma attacks, cough, pulmonary diseases could be also treated with guava teas 9 and could also be useful as anti-inflammatory and hemostatic agent 10. Moreover, aqueous extracts of guava leaves were described to be effective against a number of microbial strains 11 and anti-rotavirus activity 12.
Traditional uses: Psidium guajava, is an important food crop and medicinal plant in tropical and subtropical countries is widely used like food and in folk medicine around of the world. A survey of the literature shows P. guajava is mainly known for its antispasmodic and antimicrobial properties in the treatment of diarrhoea and dysentery. It has also been used extensively as a hypo-glycaemic agent. Many pharmacological studies have demonstrated the ability of this plant to exhibit antioxidant, hepato-protective, anti-allergy, antimicrobial, antigenotoxic, antiplasmodial, cytotoxic, antispasmodic, cardioactive, anticough, antidiabetic, anti-inflammatory and antinociceptive activities, supporting its traditional uses 13.
Chemical constituents:
- Leaves: The constituents of essential oils from the leaves of Psidium guajava Linn were analyzed by GC-MS qualitatively and quantitatively. Sixty compounds of the essential oils were identified at rate 90.56%. The major components were caryophyllene (18.81%), copaene (11.80%), [1aR-(1a alpha, 4a alpha, 7 alpha, 7a beta, 7b alpha)]-decahydro-1, 1, 7-trimethyl-4-methylene-1H-cycloprop[e] azulene (10.27%), eucalyptol () 14 One new pentacyclic triterpenoid psidiumoic acid along with four known compounds beta-sitosterol, obtusol, oleanolic acid, and ursolic acid have been isolated from the leaves of Psidium guajava.
The new constituent has been characterized as 2 alpha-glycolyl-3beta-hydroxyolean-12-en-28-oic acid through 2D NMR techniques 15. Five constituents including one new pentacyclic triterpenoid guajanoic acid and four known compounds beta-sitosterol, uvaol, oleanolic acid, and ursolic acid have been isolated from the leaves of Psidium guajava. The new constituent has been characterized as 3beta-p-E-coumaroyloxy-2alpha-methoxyurs-12-en-28-oic acid 16. Guajadial, a novel caryophyllene-based meroterpenoid, was isolated from the Leaves of Psidium guajava 17. Three novel sesquiterpenoid-based meroterpenoids of psidials A-C have been isolated from the leaves of Psidium guajava L. Their complete structures were elucidated by spectral and chemical methods, and that was confirmed by single-crystal X-ray diffraction analysis. Psidial B and C showed the new skeleton of the 3, 5-diformylbenzyl phloroglucinol-coupled sesquiterpenoid 18. Four antibacterial compounds were isolated from leaves of guava (Psidium guajava L.), two new flavonoid glycosides, morin-3-O-alpha-L-lyxopyranoside and morin-3-O-alpha-L-arabopyranoside, and two known flavonoids, guaijavarin and quercetin, were identified 19. The methanolic extract of leaf contain a trace of quercetin aglycone together with five glycosides was isolated from this active fraction and identified as quercetin 3-O-alpha-L-arabinoside (guajavarin); quercetin 3-O-beta-D-glucoside (isoquercetin); quercetin 3-O-beta-D-galactoside (hyperin); quercetin 3-O-beta-L-rhamnoside (quercitrin) and quercetin 3-O-gentobioside 20.
Two triterpenoids, 20 beta-acetoxy-2 alpha, 3 beta-dihydroxyurs-12-en-28-oic acid (guavanoic acid), and 2 alpha, 3 beta-dihydroxy-24-p-z-coumaroyloxyurs-12-en-28-oic acid (guavacoumaric acid), along with six known compounds 2 alpha-hydroxyursolic acid, jacoumaric acid, isoneriucoumaric acid, asiatic acid, ilelatifol D and beta-sitosterol-3-O-beta-D-glucopyranoside, have been isolated from the leaves of Psidium guajava 21. Two new triterpenoids, guajavolide (2 alpha, 3 beta, 6 beta, 23-tetrahydroxyurs-12-en-28, 20 beta-olide) and guavenoic acid (2 alpha,3 beta, 6 beta, 23-tetrahydroxyurs-12, 20 (30)-dien-28-oic acid) along with one known triterpene oleanolic acid were isolated from the fresh leaves of Psidium guajava 22. Two triterpenoids, 20 β- acetoxy- 2α, 3β-dihydroxyurs- 12- en- 28- oic acid (guavanoic acid), and 2α, 3 β- dihydroxy- 24- p- z-coumaroyloxyurs- 12- en- 28- oic acid (guavacoumaric acid), along with six known compounds 2α-hydroxyursolic acid, jacoumaric acid, isoneriucoumaric acid, asiatic acid, ilelatifol D and β- sitosterol-3-O-β- D- glucopyranoside, have been isolated from the leaves of Psidium guajava 23.
Ursolic acid
(i) α copaene
(ii) β copaene
- Seeds: Ten phenolic and flavonoid compounds including one new acylated flavonol glycoside were isolated from Psidium gauijava The new compound isolated was quercetin-3-O-beta-D-(2"-O-galloyl glucoside)-4'-O-vinylpropionate 24. Diguajadial is a new meroterpenoid, which is symmetric homo dimeric ether with two guajadial units was isolated from Psidium guajava seeds 25. Guava seeds found to contain phenolic fraction with results varying from 0.380 to 1.738% (w/w) 26. The high-performance liquid chromatography chromatogram showed that the Guava 'Horana red' variety contained almost exclusively lycopene, with a small amount of lutein, beta-carotene and beta-cryptoxanthin 27.
- Flesh: Sixteen carotenoids were isolated from the flesh of Brazilian red guavas. Their structures were established by means of UV-visible, 400 and 500 MHz 1H NMR, 120 and 125 MHz 13C NMR, mass spectra, and circular dichroism spectra. Thirteen of the carotenoids identified are reported as guava carotenoids for the first time 28.
- Fruits: The ripe edible fruits of Psidium guajava L. was phytochemically investigated, three benzophenone glycosides, 2, 6- dihydroxy- 3, 5- dimethyl-4- O- beta- D- glucopyranosyl-benzophenone, 2, 6- dihydroxy- 3- methyl-4-O-(6''- O- galloyl- beta- D- glucopyranosyl)- benzophenone, 2, 6-dihydroxy- 3, 5- dimethyl- 4- O- (6''-O-galloyl- beta- D- glucopyranosyl)-benzophenone were isolated by means of chromatography 29. A water-soluble polysaccharide was isolated from hot aqueous extracts of fruits of Psidium guajava. The polysaccharide was found to contain 2-O-methyl-l-arabinose, 2-O-acetyl-D-galactose, and D-methyl galacturonate in a molar ratio of approximately 1:1:1 30. Immature fruits and those in their intermediate stage of maturation, were predominantly the aldehydes such as (E)-2-hexenal and (Z)-3-hexenal. In mature fruits, esters like Z-3-hexenyl acetate and E-3-hexenyl acetate and sesquiterpenes caryophyllene, α-humulene and β-bisabollene are present 31. Characterization of the aromatic profile in commercial guava essence and fresh fruit puree by GC-MS yielded a total of 51 components quantified. Commercial essence was characterized to present a volatile profile rich in components with low molecular weight, especially alcohols, esters, and aldehydes, whereas in the fresh fruit puree terpenic hydrocarbons and 3-hydroxy-2-butanone were the most abundant components.
In the olfactometric analyses totals of 43 and 48 aroma active components were detected by the panelists in commercial essence and fruit puree, respectively. New components were described for the first time as active aromatic constituents in pink guava fruit (3-penten-2-ol and 2-butenyl acetate). Principal differences between the aroma of the commercial guava essence and the fresh fruit puree could be related to acetic acid, 3-hydroxy-2-butanone, 3-methyl-1-butanol, 2,3-butanediol, 3-methylbutanoic acid, (Z)-3-hexen-1-ol, 6-methyl-5-hepten-2-one, limonene, octanol, ethyl octanoate, 3-phenylpropanol, cinnamyl alcohol, alpha-copaene, and an unknown component. (E)-2-Hexenal seems to be more significant to the aroma of the commercial essence than of the fresh fruit puree 32.
- Whole plant: An extract of whole guava, after TLC and GLC, showed the presence of two monoterpenes and nine sesquiterpenes. β-Caryophyllene comprised 95% of this fraction 33. Bioautography directed chromatographic fractionation, yield biologically active compound, quercetin- 3- O- alpha- l- arabinopyranoside (guaijaverin), from crude methanol extract of P. guajava 34. Numerous tannins, polyphenolic compounds, flavonoids, pentacyclic triterpenoids, guiajaverin, quercetin, and other chemical compounds are present in the plant 35. Nine more compounds were isolated from this plant, and the structures of them were identified as ursolic acid, 2 alpha-hydroxyursolic acid, 2 alpha-hydroxyoleanolic acid, morin- 3- O- alpha- L- arabopyranoside, quercetin, hyperin, myricetin- 3- O- beta- D- glucoside, quercetin- 3- O- beta- D-glucuronopyranoside, 1- O- galloyl- beta- D- glucose36.
Pharmacological Activity:
PHARMACOLOGICAL ACTIVITY | DETAILS | REFERENCE |
Antioxidant activity
|
The extracts from distilled water, 65% ethanol and 95% ethanol respectively showed effects on scavenging hydroxyl radicals and inhibiting lipid peroxidation in the dose-dependent manner, had 50% effective concentration (EC50) on scavenging hydroxyl radicals of 0.63, 0.47 and 0.58g/L, had EC50 on inhibiting lipid peroxidation of 0.20, 0.035, 0.18g/L. | 37 |
Treatment of cough
|
The water extract of the plant at doses of 2 and 5 g/kg, p.o. decreased the frequency of cough induced by capsaicin aerosol by 35 and 54%, respectively, as compared to the control, within 10 min after injection of the extract, (P < 0.01). | 11 |
Anti-diabetic activity
|
The ethanolic stem bark extract exhibited statistically significant hypoglycaemic activity in alloxan-induced hyperglycaemic rats but was devoid of significant hypoglycaemic effect in normal and normal glucose loaded rats (OGTT). In both acute and sub-acute tests, the water extract, at an oral dose of 250 mg/kg, showed statistically significant hypoglycemic activity. | 38, 39 |
Antimicrobial activity
|
Psidium guajava aqueous bark and methanolic extracts were found to possess anti-bacterial activity. Four antibacterial compounds were isolated from leaves of guava were identified. The minimum inhibition concentration of morin-3-O-alpha-L-lyxopyranoside and morin-3-O-alpha-L-arabopyranoside was 200 microg/ml for each against Salmonella enteritidis, and 250 microg/ml and 300 microg/ml against Bacillus cereus, respectively. | 40, 19 |
Hepatoprotective activity
|
P. guajava aqueous leaf extracts (250 and 500mg/kg, po) possesses good hepatoprotective activity. | 41 |
Antidiarrhoeal activity | Psidium guajava leaf aqueous extract (PGE) (50-400 mg/kg p.o.) produced dose-dependent and significant protection of rats and mice against castor oil-induced diarrhoea, inhibited intestinal transit, and delayed gastric emptying. Like atropine (1 mg/kg, p.o.), PGE produced dose-dependent and significant antimotility effect, and caused dose-related inhibition of castor oil-induced enteropooling in the animals. Like loperamide (10 mg/kg, p.o.), PGE dose-dependently and significantly delayed the onset of castor oil-induced diarrhoea, decreased the frequency of defaecation, and reduced the severity of diarrhoea in the rodents. | 42 |
Treatment of plaque
|
The active flavonoid compound, quercetin-3-O-alpha-l-arabinopyranoside (guaijaverin) isolated from Psidium guajava demonstrated high potential antiplaque agent by inhibiting the growth of the Strep. Mutans. | 34 |
Spermatoprotective activity | The extracts of the leaves of Psidium guajava Linn. possess beneficial effects on sperm production and quality, and may thus improve the sperm parameters of infertile males with oligospermia and nonobstructive azoospermia. | 43 |
Antimutagenic activity | The water extract of P.guajava was effective in inactivating the mutagenicity of direct-acting mutagens. | 44 |
Inotropic effect | The extract from P. guajava leaves depress myocardial inotropism. | 45 |
Spasmolytic effect | The spasmolytic activity of the Psidium guajava leaf remedy is mainly due to the aglycone quercetin, present in the leaf and in the extract mainly in the form of five flavonols, and whose effect is produced when these products are hydrolyzed by gastrointestinal fluid. | 20 |
Treatment of infantile rotaviral enteritis | Psidium guajava showed good curative effect on infantile rotaviral enteritis. | 46 |
Anti- cancer activity | Aqueous extract of Psidium guajava L. budding leaves has been shown to possess anti-prostate cancer activity in a cell line model. Treatment with Psidium guajava L. budding leaves (1.5 mg/mouse/day) significantly diminished both the prostate specific antigen (PSA) serum levels and tumor size in a xenograft mouse tumor model. | 47 |
Antifungal activity | The hot water extract and the methanol extract of Psidium guajava showed high activity against Arthrinium sacchari and Chaetomium funicola strains. | 48 |
Analgesic & anti-inflammatory activity | The aqueous extract of P. guajava leaves possesses analgesic and antiinflammatory properties. The hexane, ethyl acetate and methanol extracts of Psidium guajava leaves (20,100,500 and 1250 mg/kg) exhibited mostly dose-dependent antinociceptive effects in chemical and thermal tests of analgesia. | 49,50 |
Immunomodulatory activity | Extracts derived from Psidium guajava revealed immunomodulatory activities. | 51 |
Treatment of acne | Psidium guajava leaf extracts are used in treatment of acne. | 52 |
Antiproliferative activity | Guava leaf extract has antiproliferatve activity caused by inhibition of the catalytic activity of Prostaglandin endoperoxide H synthase (PGHS) isoforms. | 53 |
Antipyretic | The methanol extract of the leaves of Psidium guajava exhibited an antipyretic effect. | 54 |
Contractile effect | Aqueous leaves extract of Psidium guajava significantly and dose-dependently (0.25-2 mg/ml) contracted aorta rings. The effect of P. guajava was to a large extent mediated by activation of alpha-adrenoceptor and to a lesser extent by acting via calcium ion channel | 55 |
Hypotensive | P. guajava leaf aqueous extract (PGE, 50-800 mg/kg i.v.) produced dose-dependent, significant reductions in systemic arterial blood pressures and heart rates of hypertensive, Dahl salt-sensitive rats. The extract causes hypotension in the mammalian experimental animal model used via cholinergic mechanisms. | 35 |
Malaria | The leaves are used as an ingredient in the preparation of fever “teas”. They are also used as a part of the pot herb used in steam treatment for malaria. The stem bark extract contained anthraquinones, flavonoids, seccoirridoids and terpenoids and was found to be effective for the treatment and/or prophylaxis of malaria. | 56 |
Oral care | In southern Nigeria the twigs are used as chew sticks and the presence of bioactive compounds comprised of saponins, tannins, flavonoids, alkaloids is responsible for their effectiveness. Chewing sticks when used without toothpaste are very efficient, effective, and reliable for cleaning teeth. The teeth of chewing sticks users are usually strong, clean, fresh, and devoid of dental plaques carries. | 57 |
CNS Activity | The leaves of the guava tree in decoction are used for spasms, epiand even for cerebral affections. | 58 |
Conjunctivitis | Flowers are also used as a poultice for conjunctivitis. | 59 |
Vaginal Disorders | The leaves of the guava tree in decoction are recommended fouterine haemorrhage. The same decoction is used as a wash for vaginal and uterine problems, and especially where an astringent remedy is needed. | 58 |
Rheumatism | The pounded leaves in India are used for rheumatism. | 59 |
- Antioxidant activity: The extracts from distilled water, 65% ethanol and 95% ethanol respectively showed effects on scavenging hydroxyl radicals and inhibiting lipid peroxidation in the dose-dependent manner, had 50% effective concentration (EC50) on scavenging hydroxyl radicals of 0.63, 0.47 and 0.58g/L, had EC50 on inhibiting lipid peroxidation of 0.20, 0.035, 0.18g/L. 37
- Treatment of cough: The water extract of the plant at doses of 2 and 5 g/kg, p.o. decreased the frequency of cough induced by capsaicin aerosol by 35 and 54%, respectively, as compared to the control, within 10 min after injection of the extract, (P < 0.01) 11.
- Anti-diabetic activity: The ethanolic stem bark extract exhibited statistically significant hypoglycaemic activity in alloxan-induced hyperglycaemic rats but was devoid of significant hypoglycaemic effect in normal and normal glucose loaded rats (OGTT) 38. In both acute and sub-acute tests, the water extract, at an oral dose of 250 mg/kg, showed statistically significant hypoglycemic activity
- Antimicrobial activity: Psidium guajava aqueous bark and methanolic extracts were found to possess anti-bacterial activity 40. Four antibacterial compounds were isolated from leaves of guava were identified. The minimum inhibition concentration of morin-3-O-alpha-L-lyxopyranoside and morin-3-O-alpha-L-arabopyranoside was 200 microg/ml for each against Salmonella enteritidis, and 250 microg/ml and 300 microg/ml against Bacillus cereus, respectively 19.
- Hepatoprotective activity: guajava aqueous leaf extracts (250 and 500mg/kg, po) possesses good hepatoprotective activity 41.
- Antidiarrhoeal activity: Psidium guajava leaf aqueous extract (PGE) (50-400 mg/kg p.o.) produced dose-dependent and significant protection of rats and mice against castor oil-induced diarrhoea, inhibited intestinal transit, and delayed gastric emptying. Like atropine (1 mg/kg, p.o.), PGE produced dose-dependent and significant antimotility effect, and caused dose-related inhibition of castor oil-induced enteropooling in the animals. Like loperamide (10 mg/kg, p.o.), PGE dose-dependently and significantly delayed the onset of castor oil-induced diarrhoea, decreased the frequency of defaecation, and reduced the severity of diarrhoea in the rodents 42.
- Treatment of plaque: The active flavonoid compound, quercetin- 3- O- alpha- l-arabinopyranoside (guaijaverin) isolated from Psidium guajava demonstrated high potential antiplaque agent by inhibiting the growth of the Strep. mutans 34 .
- Spermatoprotective activity: The extracts of the leaves of Psidium guajava possess beneficial effects on sperm production and quality, and may thus improve the sperm parameters of infertile males with oligospermia and nonobstructive azoospermia 43.
- Antimutagenic activity: The water extract of guajava was effective in inactivating the mutagenicity of direct-acting mutagens 44.
- Inotropic effect: The extract from P. guajava leaves depress myocardial inotropism 45.
- Spasmolytic effect: The spasmolytic activity of the Psidium guajava leaf remedy is mainly due to the aglycone quercetin, present in the leaf and in the extract mainly in the form of five flavonols, and whose effect is produced when these products are hydrolyzed by gastrointestinal fluid 20.
- Treatment of infantile rotaviral enteritis: Psidium guajava showed good curative effect on infantile rotaviral enteritis 46.
- Anti- cancer activity: Aqueous extract of Psidium guajava budding leaves has been shown to possess anti-prostate cancer activity in a cell line model. Treatment with Psidium guajava L. budding leaves (1.5 mg/mouse/day) significantly diminished both the prostate specific antigen (PSA) serum levels and tumor size in a xenograft mouse tumor model 47.
- Antifungal activity: The hot water extract and the methanol extract of Psidium guajava showed high activity against Arthrinium sacchari and Chaetomium funicola strains 48.
- Analgesic & anti-inflammatory activity: The aqueous extract of guajava leaves possesses analgesic and antiinflammatory properties 49. The hexane, ethyl acetate and methanol extracts of Psidium guajava leaves (20,100,500 and 1250 mg/kg) exhibited mostly dose-dependent antinociceptive effects in chemical and thermal tests of analgesia 50.
- Immunomodulatory activity: Extracts derived from Psidium guajava revealed immunomodulatory activities 51.
- Treatment of acne: Psidium guajava leaf extracts are used in treatment of acne 52.
- Antiproliferative activity: Guava leaf extract has antiproliferatve activity caused by inhibition of the catalytic activity of Prostaglandin endoperoxide H synthase (PGHS) isoforms 53.
- Antipyretic: The methanol extract of the leaves of Psidium guajava exhibited an antipyretic effect 54.
- Contractile effect: Aqueous leaves extract of Psidium guajava significantly and dose-dependently (0.25-2 mg/ml) contracted aorta rings. The effect of guajava was to a large extent mediated by activation of alpha-adrenoceptor and to a lesser extent by acting via calcium ion channel 55.
- Hypotensive: guajava leaf aqueous extract (PGE, 50-800 mg/kg i. v.) produced dose-dependent, significant reductions in systemic arterial blood pressures and heart rates of hypertensive, Dahl salt-sensitive rats. The extract causes hypotension in the mammalian experimental animal model used via cholinergic mechanisms 35.
- Malaria: The leaves are used as an ingredient in the preparation of fever “teas”. They are also used as a part of the pot herb used in steam treatment for malaria. The stem bark extract contained anthraquinones, flavonoids, seccoirridoids and terpenoids and was found to be effective for the treatment and/or prophylaxis of malaria56.
- Oral care: In southern Nigeria the twigs are used as chew sticks and the presence of bioactive compounds comprised of saponins, tannins, flavonoids, alkaloids is responsible for their effectiveness. Chewing sticks when used without toothpaste are very efficient, effective, and reliable for cleaning teeth. The teeth of chewing sticks users are usually strong, clean, fresh, and devoid of dental plaques carries 57.
- CNS Activity: The leaves of the guava tree in decoction are used for spasms, epiand even for cerebral affections 58.
- Conjunctivitis: Flowers are also used as a poultice for conjunctivitis 59.
- Vaginal Disorders: The leaves of the guava tree in decoction are recommended fouterine haemorrhage. The same decoction is used as a wash for vaginal and uterine problems, and especially where an astringent remedy is needed 58.
- Rheumatism: The pounded leaves in India are used for rheumatism 59.
Safety Data: The LD50 of the aqueous extract was >5 g/kg, p. o. 60.
CONCLUSION: Major thrust by whole of the pharmaceutical industry is focused towards design and development of new innovative/indigenous plant based drugs through investigation of leads from traditional system of medicine 61. In recent years, ethno-botanical and traditional uses of natural compounds, especially of plant origin received much attention as they are well tested for their efficacy and generally believed to be safe for human use. It is best classical approach in the search of new lead molecules for management of various diseases. Thorough screening of literature available on Psidium guajava depicted the fact that it is a popular remedy among the various ethnic groups, vaidhyas, hakims and Ayurvedic practitioners for cure of ailments. It is needed to explore this plant very thoroughly and researchers are exploring the therapeutic potential of this plant.
REFERENCES:
- Pathak RK, Ojha, CM: Genetic resources of guava, Vol. I, Fruit Crops, Part 1, In; Advance in Horticulture [C]. Chadha KL, Pareek OP, editorss, Malhotra Publishing House, New Delhi, 1993, pp. 143–147.
- Bailey LH: The standard encyclopedia of horticulture [C]. Vol. II. Macmillan Co., New York. 1960, pp. 1415.
- Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH: Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006; 19: 669–675.
- Koo MH, Mohamed S: Flavonoid (myricetin, quercetin, kaempferol, luteolin and apigenin) content of edible tropical plants. J Agric Food Chem 2001; 49: 3106–3112.
- Rahman M, Begum K, Begum M, Faruque CAA: Correlation and path analysis in guava. Bangladesh J. Agril. Res. 2003; 28 (1): 93–98.
- Drurey CH: The useful plants of India [M]. International book distributors, Dheradun. 1985, pp. 512.
- Lutterodt GD: Inhibition of Microlax-induced experimental diarrhea with narcotic-like extracts of Psidium guajava leaf in rats. Journal of Ethno pharmacology 1992; 37(2): 151-157.
- Lutterodt GD, Maleque A : Effects on mice locomotor activity of a narcotic-like principle from Psidium guajava Journal of Ethnopharmacology 1988; 24(2-3): 219-231.
- Batick MJ: Ethnobotany of Palms in the Neotropics. In; Prance GT, Kallunki JA, editors, Advances in Economic Botany: Ethnobotany in the Neotropics. New York Botanical Garden, New York, USA, 1984, pp. 9-23.
- Liu HN: Chinese Medicinal Herbs of Hong Kong. Vol. 2. Hang Chiewing As Kwang, Hong Kong. 1988, pp. 104-105.
- Chulasiri M, Suthienkul O, Pavaro C, Wongkrajang Y: Herbal extracts for diarrheal treatment: antibacterial activity in vitro. Journal of Public Health 1986; 16: 21-35.
- Goncalves JLS, Lopes RC, Oliveira DB, Costa SS, Miranda MMFS, Romano MTV et al.: In vitro anti-rotavirus activity of some medicinal plants used in Brazil against diarrhea. Journal of Ethnopharmacology 2005; 99(3): 403-407.
- Gutiérrez RM, Mitchell S, Solis RV: Psidium guajava: a review of its traditional uses, phytochemistry and pharmacology. J Ethnopharmacol. 2008; 117(1): 1-27.
- Li J, Chen F, Luo J: GC-MS analysis of essential oil from the leaves of Psidium guajava Zhong Yao Cai. 1999; 22(2): 78-80.
- Begum S, Ali SN, Hassan SI, Siddiqui BS: A new ethylene glycol triterpenoid from the leaves of Psidium guajava. Nat Prod Res. 2007; 21(8): 742-748.
- Begum S, Hassan SI, Ali SN, Siddiqui BS: Chemical constituents from the leaves of Psidium guajava. Nat Prod Res. 2004; 18(2): 135-140.
- Yang XL, Hsieh KL, Liu JK: Guajadial: an unusual meroterpenoid from guava leaves Psidium guajava. Org Lett. 2007; 9(24): 5135-5138.
- Fu HZ, Luo YM, Li CJ, Yang JZ, Zhang DM. Psidials A-C: Three Unusual Meroterpenoids from the Leaves of Psidium guajava Org Lett. 2010 (In press).
- Arima H, Danno G: Isolation of antimicrobial compounds from guava (Psidium guajava) and their structural elucidation. Biosci Biotechnol Biochem. 2002; 66(8): 1727-1730.
- Lozoya X, Meckes M, Abou-Zaid M, Tortoriello J, Nozzolillo C, Arnason JT: Quercetin glycosides in Psidium guajava leaves and determination of a spasmolytic principle. Arch Med Res. 1994; 25(1): 11-15
- Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH: Triterpenoids from the leaves of Psidium guajava. Phytochemistry 2002; 61(4): 399-403.
- Begum S, Hassan SI, Siddiqui BS: Two new triterpenoids from the fresh leaves of Psidium guajava. Planta Med. 2002; 68(12): 1149-1152.
- Begum S, Hassan SI, Siddiqui BS, Shaheen F, Ghayur MN and Anwar H. Gilani : Triterpenoids from the leaves of Psidium guajava. Phytochemistry 2002; 61(4): 399-403.
- Michael HN, Salib JY, Ishak MS: Acylated flavonol glycoside from Psidium gauijava seeds. Pharmazie 2002; 57(12): 859-860.
- Xiao-Long YANG, Kun-Lung HSIEH, Ji-Kai LIU: Diguajadial: A Dimer of the Meroterpenoid from the Leaves of Psidium guajava (Guava). Chinese Journal of Natural Medicines 2008; 6(5): 333-335.
- Castro-Vargas HI, Rodríguez-Varela LI, Ferreira SRS, Parada-Alfonso F: Extraction of phenolic fraction from guava seeds (Psidium guajava) using supercritical carbon dioxide and co-solvents. The Journal of Supercritical Fluids 2010; 51(3): 319-324.
- Chandrika UG, Fernando KS, Ranaweera KK: Carotenoid content and in vitro bioaccessibility of lycopene from guava (Psidium guajava) and watermelon (Citrullus lanatus) by high-performance liquid chromatography diode array detection. Int J Food Sci Nutr. 2009; 60(7): 558-566.
- Mercadante AZ, Steck A, Pfander H: Carotenoids from guava (Psidium guajava): isolation and structure elucidation. J Agric Food Chem. 1999; 47(1): 145-151.
- Shu J, Chou G, Wang Z: Two new benzophenone glycosides from the fruit of Psidium guajava Fitoterapia 2010. (In press).
- Mandal S, Sarkar R, Patra P, Nandan CK, Das D, Bhanja SK et al.: Structural studies of a heteropolysaccharide (PS-I) isolated from hot water extract of fruits of Psidium guajava (Guava). Carbohydr Res. 2009; 344(11): 1365-1370.
- Soares FD, Pereira T, Marques MOM, Monteiro AR: Volatile and non-volatile chemical composition of the white guava fruit (Psidium guajava) at different stages of maturity. Food Chemistry 2007; 100(1): 15-21.
- Jordán MJ, Margaría CA, Shaw PE, Goodner KL: Volatile components and aroma active compounds in aqueous essence and fresh pink guava fruit puree (Psidium guajava) by GC-MS and multidimensional GC/GC-O. J Agric Food Chem. 2003; 51(5): 1421-1426.
- Charles W. Wilson III, Philip E Shaw: Terpene hydrocarbons from Psidium guajava. Phytochemistry 1978; 17(8): 1435-1436
- Prabu GR, Gnanamani A, Sadulla S: Guaijaverin -- a plant flavonoid as potential antiplaque agent against Streptococcus mutans. J Appl Microbiol. 2006; 101(2): 487-495.
- Ojewole JAO: Hypoglycemic and hypotensive effects of Psidium guajava (Myrtaceae) leaf aqueous extract. Methods Find Exp Clin Pharmacol. 2005; 27(10): 689-695.
- Fu H, Luo Y, Zhang D: Studies on chemical constituents of leaves of Psidium guajava. Zhongguo Zhong Yao Za Zhi. 2009; 34(5): 577-579.
- Wang B, Jiao S, Liu H, Hong J: Study on antioxidative activities of Psidium guajava Linn leaves extracts .Wei Sheng Yan Jiu. 2007; 36(3): 298-300.
- Mukhtar HM, Ansari SH, Bhat ZA, Naved T, Singh P: Antidiabetic activity of an ethanol extract obtained from the stem bark of Psidium guajava (Myrtaceae). Pharmazie 2006; 61(8): 725-727.
- Mukhtar HM, Ansari SH, Ali M, Naved T, Bhat ZA: Effect of water extract of Psidium guajava leaves on alloxan-induced diabetic rats. Pharmazie 2004; 59(9): 734-735.
- Abdelrahim SI, Almagboul AZ, Omer ME, Elegami A: Antimicrobial activity of Psidium guajava Fitoterapia 2002; 73(7-8): 713-715.
- Roy CK, Kamath JV, Asad M: Hepatoprotective activity of Psidium guajava leaf extract. Indian J Exp Biol. 2006; 44(4): 305-311.
- Ojewole JA, Awe EO, Chiwororo WD: Antidiarrhoeal activity of Psidium guajava (Myrtaceae) leaf aqueous extract in rodents. J Smooth Muscle Res. 2008; 44(6): 195-207.
- Akinola OB, Oladosu OS, Dosumu OO. Ethanol extract of the leaves of Psidium guajava Linn enhances sperm output in healthy Wistar rats. Afr J Med Med Sci. 2007; 36(2): 137-140.
- Grover IS, Bala S: Studies on antimutagenic effects of guava (Psidium guajava) in Salmonella typhimurium. Mutat Res. 1993; 300(1): 1-3.
- Conde Garcia EA, Nascimento VT, and Santiago Santos AB: Inotropic effects of extracts of Psidium guajava (guava) leaves on the guinea pig atrium. Braz J Med Biol Res. 2003; 36(5): 661-668.
- Wei L, Li Z, Chen B: Clinical study on treatment of infantile rotaviral enteritis with Psidium guajava Zhongguo Zhong Xi Yi Jie He Za Zhi. 2000; 20(12): 893-895.
- Chen KC, Peng CC, Chiu WT, Cheng YT, Huang GT, Hsieh CL et al.: Action mechanism and signal pathways of Psidium guajava aqueous extract in killing prostate cancer LNCaP cells. Nutr Cancer 2010; 62(2): 260-270.
- Sato J, Goto K, Nanjo F, Kawai S, Murata K: Antifungal activity of plant extracts against Arthrinium sacchari and Chaetomium funicola. J Biosci Bioeng. 2000; 90(4): 442-446.
- Ojewole JA: Antiinflammatory and analgesic effects of Psidium guajava (Myrtaceae) leaf aqueous extract in rats and mice. Methods Find Exp Clin Pharmacol. 2006; 28(7): 441-446.
- Shaheen HM, Ali BH, Alqarawi AA, Bashir AK: Effect of Psidium guajava leaves on some aspects of the central nervous system in mice. Phytother Res. 2000; 14(2): 107-111.
- Kaileh M, Berghe WV, Boone E, Essawi T, Haegeman G: Screening of indigenous Palestinian medicinal plants for potential anti-inflammatory and cytotoxic activity. J Ethnopharmacol. 2007; 113(3): 510-516.
- Qadan F, Thewaini AJ, Ali DA, Afifi R, Elkhawad A, Matalka KZ: The antimicrobial activities of Psidium guajava and Juglans regia leaf extracts to acne-developing organisms. Am J Chin Med. 2005; 33(2): 197-204.
- Kawakami Y, Nakamura T, Hosokawa T, Suzuki-Yamamoto T, Yamashita H, Kimoto M et al.: Antiproliferative activity of guava leaf extract via inhibition of prostaglandin endoperoxide H synthase isoforms. Prostaglandins, Leukotrienes and Essential Fatty Acids 2009; 80(5-6): 239-245.
- Olajide OA, Awe SO, Makinde JM: Pharmacological studies on the leaf of Psidium guajava. Fitoterapia 1999; 70(1): 25-31.
- Olatunji-Bello II, Odusanya AJ, Raji I, Ladipo CO: Contractile effect of the aqueous extract of Psidium guajava leaves on aortic rings in rat. Fitoterapia 2007; 78(3): 241-243.
- Nundkumar N, Ojewole JA: Studies on the antiplasmodial properties of some South African medicinal plants used as antimalarial remedies in Zulu folk medicine. Methods Find Exp Clin Pharmacol. 2002 Sep; 24(7):397-401.
- Okwu, D. E.; Ekeke, O: Phytochemical screening and mineral composition of chewing sticks in South Eastern Nigeria. Global Journal of Pure and Applied Sciences 2003; 9(2):235-238.
- Ticzon, Romeo: Ticzon Herbal Medicine Encyclopedia. 1997. Romeo R. Ticzon Publishing, Philippines. ISBN No. 97191-7231-2.
- Ayensu, E.S.: Medicinal Plants of West Africa. 1978. Reference Publications, Algonac, Michigan. ISBN No. 0-917256-07-7.
- Pranee Jaiarj, Paranee Khoohaswan, Yuwadee Wongkrajang, Penchom Peungvicha, Potjanee Suriyawong, Saraya, M. L. S., Orawan Ruangsomboon : Anticough and antimicrobial activities of Psidium guajava leaf extract. Journal of Ethnopharmacology 1999; 6(2):203-212.
- Patwardhan B, Ashok DBV, Chorghade M: Ayurveda and natural products drug discovery. Current Science 2004; 80(6): 789-799.
Article Information
2
9-19
586 kB
4591
English
IJPSR
Payal Mittal*, Vikas Gupta, Gurpreet Kaur, Ashish K Garg and Amarjeet Singh
Akal College of Pharmacy and Technical Education, Mastuana Sahib, Sangrur, India
payal_mittal84@yahoo.com
28 May, 2010
14 July, 2010
20 August, 2010
http://dx.doi.org/10.13040/IJPSR.0975-8232.1(9-S).9-19
15 September, 2010