PRELIMINARY PHYTOCHEMICAL INVESTIGATION, IN-VITRO ANTIOXIDANT, IN-VITRO α-AMYLASE INHIBITION, IN-VITRO AND IN-VIVO ANTICATARACT ACTIVITY OF ETHYLACETATE ROOT EXTRACT OF ABUTILON INDICUMAbstract
The present study aims to evaluate the in-vitro antioxidant, in-vitro α-amylase inhibition, in-vitro and in-vivo anti-cataract potential of ethyl acetate root extract of Abutilon indicum. Antidiabetic activity was evaluated against alpha-amylase by spectrophotometric assays. DPPH determined antioxidant activity, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging assay. Different extract concentrations were made using dimethyl sulfoxide (DMSO) and subjected to an α-amylase inhibitory assay. The in-vitro anticataract potential of Abutilon indicum was determined using glucose-induced cataractous goat eye lens. In the in-vivo group, cataract was induced in rats by 30% galactose diet alone (control) or with the addition of Abutilon indicum (treated group). The results indicate the dose-dependent in-vitro antioxidant activity against DPPH, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging comparable with that of standard Ascorbic acid and also appreciable α-amylase inhibitory activity with IC50 values comparable with that of standard Acarbose. An in-vitro study was conducted, which reported that the lens group treated with the plant extract (500μg/ml) exhibited a reduction in opacity compared to the lens in the negative control. The study of the anti-cataract potential of Abutilon indicum exhibited an increase in total protein content, aldose reductase inhibition activity and a decrease in the malondialdehyde level compared to the negative control. Abutilon indicum can delay the onset and progression of cataracts in an experimental rat model of Glucose-induced cataracts in-vitro and galactose-induced cataracts in-vivo.
G. V. N. Kiranmayi *, P. Sirisha and R. Sanjana
Faculty of Pharmacy, Dr. M. G. R. Educational and Research Institute, Deemed to be University, Chennai, Tamil Nadu, India.
03 June 2022
01 August 2022
03 August 2022
01 February 2023