PRELIMINARY QUALITATIVE PHYTOCHEMICAL, PHYSICO-CHEMICAL AND PHYTO-PHARMACOGNOSTICAL STANDARDISATION EVALUATION OF THE PULP (WITHOUT SEED), PERICARP, LEAVES AND SEED OF AEGLE MARMELOS L. (BAEL).Abstract
Aegle marmelos L. (Bael), is a commonly occurring herb which has biomedical potency to treat many types of diseases such as diarrhea, hepatoprotective, epilepsy, stomachalgia, digestion related problems etc. The different parts of Aegle marmelos (L.) were collected, dried and coarsely powdered. The present study deals with the physio-chemical constants standardization parameters such as pH, solubility including water and methanolic extraction, total ash, acid insoluble ash, water soluble ash, extractive values, dry and wet particle size, foaming index, swelling index, foreign matters, loss of drying (LOD) and acidity were observed. Fluorescence analysis was performed for study of behavioral characters of the pulp without seed, pericarp, leaf and seed powder treated with chemical reagents. Extractive values of powder with solvents such as water and methanol were determined. Macroscopical characters help in identifying the fruit, pulp, pericarp, leaf and seed. Anatomical observations showed the presence of palisade cell, trichomes, mesophyll cell, collenchymas, xylem, phloem, pith, upper epidermal cells and lower epidermal cells with stomata. Preliminary qualitative phytochemical screening reported the presence of carbohydrates, protein, alkaloids, tannins, phenolic compounds, starch, steroids, terpenoids, glycosides, amino acids, reducing sugars, saponins, lignin, fat and flavanoids. The reported phytoconstitutents of the plant Aegle marmelos (L.) may be responsible for the pharmacological and pharmaceutical activities. Microbial limit assay were performed using standard textual methods. The qualitative screenings along with primary HPTLC fingerprinting were performed was showed the Rf values at 254 nm with their respective UV-visible spectrum wavelengths scanned in between 200 to 400 nm. The results obtained about traditional information which will further increase the usage of this plant and evoke towards the scanning of the plants. These studies will provide immense value in the botanical referential information for the correct identification and standardization of the drug in a crude form.
S. L. Shakyawar *, M. A. Rahman, S. Sundaram and S. Alok
Centre of Biotechnology (CBT), Science Faculty, University of Allahabad, Prayagraj (Allahabad), Uttar Pradesh, India.
01 March 2020
21 May 2020
25 May 2020
01 July 2020