PREVALENCE OF -56C/T REGULATORY POLYMORPHISM IN INTERFERON-γ RECEPTOR 1 PROMOTER REGION KNOWN TO BE POTENTIAL RISK FACTOR FOR HEPATITIS B VIRUS INFECTION IN NORTH INDIAN HEALTHY POPULATION
AbstractInterferon-γ (IFN-γ), a defense molecule, mediates its response against viral infections like Hepatitis B virus by binding to interferon gamma receptor 1 (IFNGR1) on cytotoxic T lymphocytes (CTLs). Hence, interferon gamma receptor 1 (IFNGR1) gene expression is vital in the eradication of the HBV virus. It has been demonstrated by some studies that -56C/T SNPs in IFNGR1 gene promoter region can be correlated with HBV-infection resistance or susceptibility. It has also been shown that -56C SNP has protective effect against HBV infection in comparison to -56T SNP. This motivate us to find out the percentage prevalence of -56C/T SNPs in our healthy population. Genomic DNA was extracted using standard phenol-chloroform method from healthy blood donors. PCR was performed to amplify the 491 bp region of IFNGR1 gene promoter region and digested with AfeI restriction enzyme to observe the -56C/T SNPs. Allele ‘T’ has been observed as the overall dominant allele (65.71%) in our both male (71.79%) and female (58.06%) healthy population.
Article Information
47
3112-16
379
1040
English
IJPSR
Vikas Pahal *, Rohit Randhawa and Krishan Swaroop Dadhich
Department of Microbiology, Dolphin (PG) College of Science and Agriculture, Chunnikalan, F.G. Sahib, Punjab, India
vikaspahal3@gmail.com
08 February, 2016
16 March, 2016
15 May, 2016
10.13040/IJPSR.0975-8232.7(7).3112-16
01 July 2016
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