PROTECTIVE POTENTIAL OF BUCHANANIA LANZAN OIL IN ORGANOPHOSPHATE -INDUCED MALE REPRODUCTIVE TOXICITY: AN EXPERIMENTAL STUDY

The purpose of this research was to examine the harmful effects of Organophosphate (OP) on reproduction as well as the putative antioxidant agent Buchanania lanzan Oil (BLO potential)’s protective properties. A total of 32 male Wistar rats were divided into four groups of eight each: the control group (CRL), the Organophosphate group (OP, 27 mg/kg), the Buchanania lanzan oil group (BLO, 0.5 ml/kg), and the combination group (OP+ BLO). For 45 consecutive days, the test animals received gavage doses (five days per week). We looked at body weight gain, weights of the reproductive organs, sperm characteristics, testosterone levels, and levels of thiobarbituric acid-reactive substances (TBARS). According to the data, OP, considerably (p<0.001) reduced both the rate of body weight gain and the absolute weights of reproductive organs (testes, epididymis, and seminal vesicles). Moreover, significant modifications in semen at least (p< 0.01) A drop in spermatid number, sperm count, sperm motility, and testosterone level, with an increase in aberrant and dead sperm and TBARS level were seen in the OP, group. BLO therapy alone has been shown to enhance spermatogenesis, increasing spermatid counts and seminal vesicle weights significantly (p<0.001). On the other hand, co-administration of BLO and OP, can more effectively and selectively modify the negative effects of OP on the weights of the reproductive organs, the quality of the semen, the levels of testosterone, and the TBARS (at least p< 0.001). This indicates the protective function of BLO against reproductive toxicity caused by OP, its antioxidant qualities, and capacity to lower TBARS levels, as demonstrated in this work, may be the cause of the induced reproductive damage.