QUALITATIVE AND QUANTITATIVE ESTIMATION OF GALLIC ACID AND ASCORBIC ACID IN POLYHERBAL TABLETS
AbstractAn HPTLC method was developed for Qualitative and Quantitative Estimation of Gallic Acid and Ascorbic Acid in Polyherbal Tablets (Amalant and Sookshma Triphala Tablet) containing Embelica Officinalis, using precoated HPTLC silica gel 60 F254 as Stationary Phase and Mobile Phase for Gallic Acid is Toluene: Ethyl Acetate: Formic Acid (6:3:1v/v/v) and for Ascorbic Acid is Ethyl Acetate: Acetic Acid Glacial: Formic Acid: Water (6:1:1:2v/v/v/v). Detection and Quantification were performed densitometrically at λmax 254nm for Gallic Acid and Ascorbic Acid. The Standard Rf values of Gallic Acid and Ascorbic Acid are 0.34±0.01 and 0.60±0.01 respectively. The total peak areas of the Standards [Gallic Acid and Ascorbic Acid] and the corresponding peak areas of extracts were compared and the Gallic Acid content were estimated to be 3.01, 4.89% and 5.01% from Amla Powder, Amalant Tablet and Sookshma Triphala Tablet and the Ascorbic Acid were estimated to be 11.56%, 28.94%, 31.79% from Amla Powder, Amalant Tablet and Sookshma Triphala Tablet
Article Information
24
2394-2398
692
3114
English
Ijpsr
N. V. Patel* and D. R. Telange
School of Pharmacy and Technology Management, SVKM’s Narsee Monjee Institute of Management Studies, Shirpur Campus, Dist. Dhule, - 425405, Maharashtra, India
19 May, 2011
12 June, 2011
17 August, 2011
http://dx.doi.org/10.13040/IJPSR.0975-8232.2(9).2394-98
01 September, 2011
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